We narrowed to 4,337 results for: U6 gRNA

1. pCBC-DT3T4

   Plasmid

   #50592

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T3, gRNA scaffold, U6-1tplus, U6-26p, T4
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBFv-U6.2B

   Plasmid

   #138401

   Purpose

   gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

   Depositor

   Shu Kondo

   Article

   Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

   Insert

   U6-2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-2

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWT060e

   Plasmid

   #107905

   Purpose

   W2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cells

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   U6-sgRNA B (CCR5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pALuWBB

   Plasmid

   #183202

   Purpose

   dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

   Depositor

   Mitch McVey

   Insert

   w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
   

   Use

   CRISPR; Recombines at attp landing site in fly ge…

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6-3

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-EEA-g1-PGK-Puro

   Plasmid

   #102908

   Purpose

   PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GG-EBNA-EEA-guide1-PGK-Puro

   Plasmid

   #102904

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GG-EBNA-TdT-guide1-PGK-Puro

   Plasmid

   #102903

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   TdT-guide1-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-CS-154

   Plasmid

   #166230

   Purpose

   lenti-U6-gRNA::CMVp-nCas9-PmCDA1-UGI-2A-mCherry

   Depositor

   Nozomu Yachie

   Article

   Fukushima et al Sci Rep. 2021 Dec 13;11(1):23889. doi: 1

   Inserts

   U6-gRNA EGFP targetting
   nCas9-PmCDA1-UGI-2A-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV promoter and Human U6

   Available since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDECKO-mCherry GFP

   Plasmid

   #78535

   Purpose

   Control plasmid (paired gRNAs against GFP)

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA) and U6 promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPTG-GG-sg2+sg3

    Plasmid

    #127199

    Purpose

    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA
    

    Use

    Empty sgrna plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6_(Gluc)_INT(GenPurpClon)

    Plasmid

    #68433

    Purpose

    General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct
    

    Use

    CRISPR; Cloning vector for generating int-like co…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBluescript_VEGFA-TS3_SpCas9

    Plasmid

    #107328

    Purpose

    U6 driven SpCas9 sgRNA expression for VEGFA site 3

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    VEGFA-TS3 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBluescript_VEGFA-TS3_NmCas9

    Plasmid

    #107329

    Purpose

    U6 driven NmCas9 sgRNA expression for VEGFA site 3

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    VEGFA-TS3 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-US-ECasE

    Plasmid

    #83961

    Purpose

    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

    Depositor

    Eleanor Chen

    Insert

    ERT2-spCas9-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    please see depositor comments below

    Promoter

    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TLCV2 sgAAVS1

    Plasmid

    #127099

    Purpose

    Encodes gRNA for human AAVS1.

    Depositor

    Adam Karpf

    Insert

    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide-Hygro

    Plasmid

    #139462

    Purpose

    Lentiviral vector with gRNA scaffold and hygromycin selectable marker

    Depositor

    Caroline Goujon

    Insert

    no sgRNA inserted; resistance gene: hygroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid

    #185060

    Purpose

    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445

    Depositor

    Bobby Koeleman

    Insert

    U6:pegRNA:scaffold:PBS+RT template
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-GG-OCT4-1-5-PGK-Puro

    Plasmid

    #102893

    Purpose

    PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OCT4-gRNAs-1-5-PGK-puro (POU5F1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    U6-96Ab and hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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