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pCF159_BRL

Plasmid

#225962

Purpose

CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-BRL (env protein)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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gag/pol

Plasmid

#14887

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

gag / pol

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pTargetF

Plasmid

#62226

Purpose

Constitutive expression of sgRNA without donor editing template DNA

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pij23119

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLX307 P2A GFP Luciferase

Plasmid

#193674

Purpose

Constitutive lentiviral expression of Luciferase

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

Luciferase

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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VSV.G

Plasmid

#14888

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

VSV-G Envelope

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-ASAP5-WPRE

Plasmid

#225709

Purpose

Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5

Use

AAV

Promoter

human synapsin

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

Plasmid

#183410

Purpose

piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

KRAB-dCas9-ecDHFR

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

Plasmid

#183411

Purpose

piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

Tet-on 3G

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1A

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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