Addgene

We narrowed to 18,314 results for: Tec

Showing: 2221 - 2240 of 18314 results

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BCJJ345

Plasmid

#117504

Purpose

YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtYAO

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVII-BFP

Plasmid

#162755

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mTagBFP2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MCS Lck only

Plasmid

#90411

Purpose

A control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.

Depositor

Katharina Gaus

Article

Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

Insert

Lck10+ Venus+mcherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pND003

Plasmid

#200949

Purpose

Yeast surface display destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Yeast surface display

Tags

AGA2, HA, and Myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBN449

Plasmid

#129555

Purpose

GAL4-induced expression of codon optimised FLP recombinase in C. elegans

Depositor

Peter Askjaer

Article

Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791

Insert

15xUAS

Use

Tags

FLP and mNeonGreen

Expression

Worm

Mutation

Promoter

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJMD13

Plasmid

#138653

Purpose

phiC31 integrating Streptomyces expression vector

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PermE* variant sequence from pDA1652

Available since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Fn-crRNA

Plasmid

#78958

Purpose

Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pET11b-SpyCatcher-M2e

Plasmid

#165990

Purpose

SpyCatcher-fused influenza M2 ectodomain with removable HisTag

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

SpyCatcher-M2e

Use

Tags

HisTag and SpyCatcher

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBBRBB-Ppuf843-1200-DsRed

Plasmid

#50962

Purpose

Expressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

Depositor

Claudia Schmidt-dannert

Article

Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

Insert

DsRed

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Ppuf843-1200

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PSUPER-344(hPot1)

Plasmid

#11133

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pBC2103-reci

Plasmid

#117172

Purpose

U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_arCas9 without NLS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pART (pT-2)

Plasmid

#62708

Purpose

Mammalian expression of tdTomato from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-i53

Plasmid

#119002

Purpose

Mammalian expression of i53 (53BP1) inhibitor and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

i53

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pnGFP

Plasmid

#64913

Purpose

Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells

Depositor

Huiwang Ai

Article

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Insert

pnGFP

Use

Tags

6xHis

Expression

Mammalian

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

CMV

Available since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBBRBB-Ppuf843-1200-PR

Plasmid

#50963

Purpose

Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

Depositor

Claudia Schmidt-dannert

Article

Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

Insert

Proteorhodopsin

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Ppuf843-1200

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBad33_ISTfu1_reRNA

Plasmid

#205355

Purpose

Bacterial expression of ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISTfu1 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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scFv-M-MLV-RT

Plasmid

#181809

Purpose

scFv-M-MLV RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

MMLV_RT

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJMD14

Plasmid

#138654

Purpose

phiOZJ integrating Streptomyces expression vector, intact RDF

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PermE* variant sequence from pDA1652

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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MCS Hras FI

Plasmid

#90413

Purpose

A control of MCS+ that associated with cell plasma membrane by two hydrophobic interaction at N and C terminus of the FRET pair.

Depositor

Katharina Gaus

Article

Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

Insert

Lck10+ Venus+mcherry + H-ras C terminus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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