19,627 results

1. ER-GCaMP6-150

   Plasmid

   #86918

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-150
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLminP_Luc2P_RE4

   Plasmid

   #90338

   Purpose

   NRF2 - Antioxidant response element gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   NRF2

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Mfn2-HA

   Plasmid

   #139192

   Purpose

   Expresses C-terminal HA tagged wt human Mfn2 in mammalian cells

   Depositor

   Allan Weissman

   Article

   Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

   Insert

   MFN2 (MFN2 Human)
   

   Tags

   3HA

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLeAPS-GFP-blast

   Plasmid

   #182230

   Purpose

   Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

   Depositor

   Michael Birnbaum

   Article

   Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

   Insert

   GFP-P2A-Blasticidin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAct:dCas9-VPR

   Plasmid

   #78898

   Purpose

   Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas12Max-ABE

   Plasmid

   #195335

   Purpose

   vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized dCas12Max-ABE
   

   Expression

   Mammalian

   Mutation

   N243R, E336R, D1049A

   Promoter

   CBh, CMV, hU6

   Available Since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO-GAlpha11-RLuc8

   Plasmid

   #140983

   Purpose

   Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha11-RLuc8 (GNA11 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p18sheLL

   Plasmid

   #37321

   Depositor

   John Schiller

   Article

   Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

   Insert

   HPV18L1+L2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Bxb1

   Plasmid

   #182142

   Purpose

   Expression of human codon-optimized Bxb1

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   human codon optimized Bxb1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-BRD3

    Plasmid

    #65377

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD3 (BRD3 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKD3

    Plasmid

    #45604

    Depositor

    Barry L. Wanner

    Article

    Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

    Insert

    FRT-cat-FRT
    

    Expression

    Bacterial

    Promoter

    R6K

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. NG-ABEmax

    Plasmid

    #124163

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    TadA-TadA(evo)-Cas9-NG
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid

    #135391

    Purpose

    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

    Depositor

    Fan Wang

    Insert

    TeLC-P2A-EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SV-ABAactDA

    Plasmid

    #38247

    Depositor

    Jerry Crabtree

    Article

    Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

    Inserts

    VP16/PYL1
    Gal4DBD/ABI*
    

    Tags

    Flag, Gal4DBD, HA, and VP16

    Expression

    Mammalian

    Mutation

    D143A

    Promoter

    SV40 (IRES) and SV40p

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-Runx1+23

    Plasmid

    #69602

    Purpose

    Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

    Depositor

    Owen Tamplin, Leonard Zon

    Article

    Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

    Inserts

    Runx1 +23 intronic enhancer (Runx1 Mouse)
    Mouse beta-globin minimal promoter
    

    Use

    5' gateway entry vector

    Promoter

    C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. wtTDP43tdTOMATOHA

    Plasmid

    #28205

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    HA tag and tdtomato

    Expression

    Mammalian

    Available Since

    June 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAW240

    Plasmid

    #170791

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Expression

    Yeast

    Promoter

    10B2 (P1 specific promoter)

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-MetRS-His

    Plasmid

    #124115

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionine--tRNA ligase (metG Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. miReporter-PGK

    Plasmid

    #82477

    Purpose

    microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-CFP

    Plasmid

    #13030

    Purpose

    Mammalian expression vector for cloning your gene fused to CFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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