Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 10,943 results for: NSI;

Showing: 4521 - 4560 of 10943 results

  1. pDC146

    Plasmid
    #169366
    Purpose
    Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Depositor
    Axel Brunger
    Article
    Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45
    Insert
    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Use
    Tags
    6 his tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169366-plasmid-map-sequence-id-333023

    Enlarge

  3. pCDNA3_AKAR5Δ

    Plasmid
    #119912
    Purpose
    Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do
    Insert
    AKAR5Δ
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    119912-plasmid-map-sequence-id-235127

    Enlarge

  5. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

    Enlarge

  20. pBL119-2

    Plasmid
    #104336
    Purpose
    Expresses thsR from a TetR regulated promoter under ATC control. Expresses sfGFP from a thsR regulated promoter. Expresses mCherry from a constituative promoter.
    Depositor
    Jeffrey Tabor
    Article
    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:
    Insert
    Thiosulfate response regulator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO1
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104336-plasmid-map-sequence-id-201648

    Enlarge

  25. pMM2_12

    Plasmid
    #183061
    Purpose
    Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    BM3R1-HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183061-plasmid-map-sequence-id-363441

    Enlarge

  26. pMM2_10

    Plasmid
    #183059
    Purpose
    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183059-plasmid-map-sequence-id-363439

    Enlarge

  27. pMM2_9

    Plasmid
    #183058
    Purpose
    Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    BM3R1-HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183058-plasmid-map-sequence-id-363425

    Enlarge

  28. pMM2_11

    Plasmid
    #183060
    Purpose
    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183060-plasmid-map-sequence-id-363416

    Enlarge

  33. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

    Enlarge

Showing: 4521 - 4560 of 10943 results