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pCMV-HA-hIFITM1

Plasmid

#58399

Purpose

Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM1 (IFITM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG423GAL-ccdB

Plasmid

#14149

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pLCK-mScarlet3_C1

Plasmid

#189771

Purpose

Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LCK-mScarlet3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDae079 (eMutaT7[transition])

Plasmid

#187622

Purpose

Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Publication

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Inserts

TadA-8e-T7RNAP
PmCDA1-T7RNAP

Expression

Bacterial

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pPSU150

Plasmid

#173836

Purpose

Expresses 150 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABMBPpepN

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-HRE-dUnaG

Plasmid

#124372

Purpose

UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

Depositor

Roland Friedel

Publication

Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

Insert

HRE-dUnaG

Use

Lentiviral

Tags

PEST-degron and Myc tag

Promoter

HRE (HIF responsive element 5x + CMV minimal prom…

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP12

Plasmid

#141265

Purpose

Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP4

Plasmid

#141258

Purpose

Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pCAS9counter

Plasmid

#107192

Purpose

Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

Depositor

Steve Salipante

Publication

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

Cas9
sgRNA

Expression

Bacterial

Promoter

prab17

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmScarlet_peroxisome_C1

Plasmid

#85063

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Str-KDEL_TNF-SBP-EGFP

Plasmid

#65278

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pFRT-TODestFLAGHAhFXR1

Plasmid

#48694

Purpose

Expresses hFXR1

Depositor

Thomas Tuschl

Publication

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FXR1 (isoform b) (FXR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSFFV_sfCherry3V(1-10)

Plasmid

#117483

Purpose

Lentiviral vector for constitutive expression of sfCherry3V(1-10)

Depositor

Bo Huang

Publication

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3V(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

6 substitutions (K45R, G52D, L83W, T106A, K182R, …

Promoter

SFFV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-2P-N317P

Plasmid

#196378

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Publication

Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:

Insert

S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV 3C cleavage site (before tags) C terminal on …

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only
pHAGE-FGFR1

Plasmid

#116740

Purpose

Lentiviral expression of FGFR1

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p4028 GST-E6AP

Plasmid

#8655

Depositor

Peter Howley

Publication

Oda et al Proc Natl Acad Sci U S A 1999 Aug 17;96(

Insert

E6-AP (UBE3A Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-PE6a

Plasmid

#207851

Purpose

Mammalian expression of PE6a prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6a

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p-EF1a-CreERT2-3Xflag-T2A-eBFP2

Plasmid

#170186

Purpose

This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

Depositor

Bradley Bernstein

Publication

Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

Insert

CreERT2-T2A-eBFP2

Use

Lentiviral

Promoter

EF1 alpha

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

Plasmid

#141371

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

C145A, human codon optimized

Promoter

EF1alpha

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

10 Articles

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YE1-BE4max

Plasmid

#138155

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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