We narrowed to 7,880 results for: Lif

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pHAGE-FLAG-APEX2-LGALS1

Plasmid

#175755

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS1 (LGALS1 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEF HaloTag-SYB2

Plasmid

#197282

Purpose

Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Expression

Mammalian

Mutation

Q76V and F77W

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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Sema3c(L)-AP-His

Plasmid

#72015

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21ΔRING-Box

Plasmid

#105523

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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Plxnb1(L)-Fc-His

Plasmid

#72126

Purpose

Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb1 (Plxnb1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-miniTurbo-NES-YFP-STOP-L2

Plasmid

#127359

Purpose

gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TIE-V5/HIS

Plasmid

#205612

Purpose

Expression of human TIE receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TIE receptor tyrosine kinase (TIE1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

Plasmid

#193854

Purpose

Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

5' Cbr-unc-119(+) + 5' mNeonGreen

Use

CRISPR and Cre/Lox

Expression

Worm

Promoter

Cbr-unc-119p

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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mAMCase_CatD_6xHis_pTwistCMV

Plasmid

#200228

Purpose

Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

Depositor

James S Fraser

Article

Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

Insert

Acidic Mammalian Chitinase (Chia1 Mouse)

Tags

6xHis

Expression

Mammalian

Mutation

S354F

Promoter

CMV

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Sema6d.4-AP-His

Plasmid

#72044

Purpose

Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.4 (Sema6d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-nl.mCherry-GCaMP7s

Plasmid

#172385

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

nuclear-localized mCherry + GCaMP7s

Use

Neurotropic virus

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-u(S)Cas9

Plasmid

#127383

Purpose

Expresses Cas9 at high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(S)-Cas9

Use

CRISPR

Expression

Insect

Promoter

UAST

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCCM

Plasmid

#162708

Purpose

Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-tdTomato-iCre

Plasmid

#172374

Purpose

Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

tdTomato + iCre

Use

Neurotropic virus

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSH 1-178

Plasmid

#160050

Purpose

Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSH residues 1-178 (STAMBP Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-178

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-MSI1(FL)

Plasmid

#60358

Purpose

express mouse MSI1 in mammalian cells

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (FL) (Msi1 Mouse)

Expression

Bacterial

Promoter

CMV

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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Sema4g-Fc-His

Plasmid

#72160

Purpose

Expresses the extracellular region of the Sema4G protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4g (Sema4g Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx8

Plasmid

#108136

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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