4,176 results

1. pAS1NB c Rosella I

   Plasmid

   #71245

   Purpose

   a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

   Depositor

   Mark Prescott

   Article

   Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

   Insert

   Rosella
   

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 7TGC

   Plasmid

   #24304

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-mCherry
   

   Use

   Lentiviral

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti X1 Puro DEST (694-6)

   Plasmid

   #17297

   Purpose

   3rd gen lentiviral promoter-less Gateway destination vector, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-hCRM1

   Plasmid

   #17647

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   CRM1 (XPO1 Human)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO5.sgRNA.EFS.tRFP657

   Plasmid

   #57824

   Purpose

   Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP657
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAX APP 695

   Plasmid

   #30137

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Expression

   Mammalian

   Mutation

   wild type splice variant 695

   Available Since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-MATR3

   Plasmid

   #32880

   Depositor

   Yossi Shiloh

   Article

   Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

   Insert

   MATR3 Matrin3 (MATR3 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET His6 GST TEV LIC cloning vector (2G-T)

   Plasmid

   #29707

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, GST, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJFRC7-20XUAS-IVS-mCD8::GFP

   Plasmid

   #26220

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   mCD8::GFP
   

   Expression

   Insect

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. IgK-IFN-luc

    Plasmid

    #14886

    Purpose

    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

    Depositor

    David Baltimore

    Article

    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

    Insert

    IgK-IFN (IFNB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2 Notch1 Full Length-6MT

    Plasmid

    #41728

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Full Length (Notch1 Mouse)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    L1997V; Contains murine Notch-1 protein up to ami…

    Available Since

    Dec. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-1(nsp8-nsp7)(nsp12)

    Plasmid

    #165451

    Purpose

    Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

    Depositor

    Marc Delarue

    Article

    Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

    Inserts

    nsp12
    nsp8
    nsp7
    

    Tags

    14Histidine-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCh-alpha-tubulin

    Plasmid

    #49149

    Purpose

    expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    alpha tubulin (TUBA1B Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA flag PPAR gamma

    Plasmid

    #8895

    Purpose

    Mammalian expression of flag-tagged PPAR gamma

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-TO-dCas9-3XmCherry

    Plasmid

    #64108

    Purpose

    Expression of Sp dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVSV Venus VSV-G

    Plasmid

    #36399

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    VSV Genome
    

    Promoter

    VSV promoters

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-Sos1

    Plasmid

    #32920

    Depositor

    Dafna Bar-Sagi

    Article

    Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

    Insert

    SOS1 (SOS1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-HA-USP30

    Plasmid

    #22578

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP30 (USP30 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-Tpr

    Plasmid

    #35024

    Depositor

    Larry Gerace

    Article

    Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

    Insert

    Tpr (TPR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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