We narrowed to 15,732 results for: LEA

1. B-HypaR-SpCas9

   Plasmid

   #126764

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A; N692A; M694A; Q695A; H698A; amino acids 10…

   Promoter

   CBh

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGBT9-FHIP

   Plasmid

   #198527

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FHIP (FHIP1B Human)
   

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   silent mutations in codons 353 and 651 as well as…

   Promoter

   ADH1

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24TEV-tSHIP2

   Plasmid

   #183771

   Purpose

   Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone

   Depositor

   William Kerr

   Article

   Sudan et al Front Immunol. 2022 May 4;13:830961. doi

   Insert

   SHIP2 (INPPL1 Human)
   

   Tags

   6HIS and Tobacco Etch Virus (TEV) Cleavage site

   Expression

   Bacterial

   Mutation

   includes aa18 to aa985 only

   Promoter

   T7 lac

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MSNA-c029

   Plasmid

   #175471

   Purpose

   Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.

   Depositor

   Opher Gileadi

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   L281R, H288A

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NKP9 pmRFP-N2 (719)

   Plasmid

   #61999

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf62 (CYBC1 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. NKP40 RFP (794)

   Plasmid

   #62006

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   C17orf32 (C17orf32 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   pgk

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBa-LSS-GFP-LDLR wt

   Plasmid

   #98184

   Purpose

   Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

   Insert

   LDLR (LDLR Human)
   

   Tags

   GFP and LDLR signal sequence, N-term of GFP so GF…

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta Actin

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mAzami-Green-Galectin

   Plasmid

   #102419

   Purpose

   Expresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.

   Depositor

   Geert van den Bogaart

   Article

   Dingjan et al Sci Rep. 2016 Feb 24;6:22064. doi: 10.10

   Insert

   LGALS3 (LGALS3 Human)
   

   Tags

   mAzami-green

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiCMV_HyPer7RgDAAO

   Plasmid

   #217653

   Purpose

   Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Use

   Lentiviral

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Promoter

   CMV

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pStreptagII 3C EGFR EABR

    Plasmid

    #234994

    Purpose

    For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    EGFR-EABR (EGFR Human)
    

    Tags

    Streptag-II, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHA StreptagII 3C NIS EABR

    Plasmid

    #234988

    Purpose

    For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NIS-EABR (SLC5A5 Human)
    

    Tags

    HA, Streptag-II, HRV 3C site

    Expression

    Mammalian

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid

    #97044

    Purpose

    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

    Plasmid

    #208947

    Purpose

    A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. STT3A pEGFP-N2 (1051)

    Plasmid

    #62025

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pSc1-DD

    Plasmid

    #80439

    Purpose

    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    E. coli dihydrofolate reductase

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV8-hSyn-flex-miR30-eGFP-shCrh

    Plasmid

    #132715

    Purpose

    Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    GFP-shCrh (Crh Rat)
    

    Use

    AAV

    Promoter

    Synapsin 1

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

    Plasmid

    #208951

    Purpose

    A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

    Plasmid

    #208950

    Purpose

    A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)

    Plasmid

    #208949

    Purpose

    A variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    RepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge