We narrowed to 11,123 results for: ena

1. pcDNA3.1-V5-hTEV

   Plasmid

   #65800

   Purpose

   expresses human codon-optimised TEV protease

   Depositor

   Douglas Green

   Article

   Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

   Insert

   V5-human TEV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   codon-optimised to express in mammalian cells

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTCAV-FLEx(loxP)-FlpO

   Plasmid

   #67829

   Purpose

   Cre-dependent expression of FlpO from CAV2 virus

   Depositor

   Liqun Luo

   Article

   Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

   Insert

   FlpO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-mCherry2-sgRNA (empty)

   Plasmid

   #198330

   Purpose

   Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   U6-sgRNA(F+E) empty
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC_mClover3_BSDminus_F0

   Plasmid

   #184165

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWXLd-RIEP-GpNLuc

   Plasmid

   #135935

   Purpose

   Expresses the GpNLuc LumiFluor in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   GpNLuc LumiFluor
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK-Snail

   Plasmid

   #36976

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Snail (Snai1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pMOD_C3003

   Plasmid

   #91095

   Purpose

   Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   GFP (+ BaeI site for GT donor cloning)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1749

   Plasmid

   #127983

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPIDCB2

   Plasmid

   #169900

   Purpose

   Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

   Depositor

   Richard Schneider

   Article

   Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

   Type

   Empty backbone

   Use

   Avian expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible

   Available since

   Dec. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP.R270

    Plasmid

    #138219

    Purpose

    Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mut2-RS

    Plasmid

    #182882

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

    Insert

    Mut2-RS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROP_C9_Puro_DNMT3B

    Plasmid

    #183284

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    DNMT3B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVTHM-mSox17-3xflag

    Plasmid

    #206377

    Purpose

    Expresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox17 (Sox17 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFS462

    Plasmid

    #89065

    Purpose

    his7 integration plasmid with Z3EVpr:GFP

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    

    Use

    S. pombe his7 integration vector

    Tags

    Expression

    Mutation

    Promoter

    Z3EV

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF-pIgH-ESCargo*

    Plasmid

    #140159

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRL-VinculinTS-A50I

    Plasmid

    #111831

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I) (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed vinculin alanine 50 to isoleucine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXWJ109Hg-gfp

    Plasmid

    #123148

    Purpose

    A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-32merR-t-PmerT-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, PmerT

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b-17X-PTDH

    Plasmid

    #166786

    Purpose

    Phosphite dehydrogenase for NAD(P)H regeneration

    Depositor

    Wilfred van der Donk

    Article

    Yang et al J Am Chem Soc. 2015 Oct 7;137(39):12426-

    Insert

    Phosphite dehydroenase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A196R, T201S, A328T, E352N, and C356D- please see…

    Promoter

    T7

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK1006 (pSL5)

    Plasmid

    #191001

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV miRNA control plasmid

    Plasmid

    #31508

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only