We narrowed to 6,501 results for: nls

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ptagBFP-TetR-NLS-VP16

Plasmid

#103835

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Publication

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

Plasmid

#69219

Purpose

Expresses wild type SpCas9 fused to Zif268 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Zif268 (Egr1 Mouse)

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107322

Purpose

Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to SaCas9

Promoter

CMV IE93

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH

Plasmid

#239740

Purpose

Plasmid expressing a rapamycin-inducible transcriptional activator

Depositor

Kate Galloway

Publication

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Use

Synthetic Biology

Available since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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JAB2500

Plasmid

#190379

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-BetIO::GFPint-NLS_ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Publication

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_pro35S-BetIO::GFPint-NLS_ADHt

Expression

Plant

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pWP172

Plasmid

#198796

Purpose

Co-expression plasmid for the OLL neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pseb-3-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP170

Plasmid

#198792

Purpose

Co-expression plasmid for the FLP, PVD neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PY48G10A.6-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP101

Plasmid

#198791

Purpose

Co-expression plasmid for the FLP, PVD neurons or the PHC neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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STEME-4

Plasmid

#138140

Purpose

Targeted simultaneous C-to-T and A-to-G in rice protoplasts

Depositor

Caixia Gao

Insert

wtTadA-TadA7.10-APOBEC3A-nCas9-2X(T2A-UGI-NLS)

Expression

Plant

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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STEME-3

Plasmid

#138139

Purpose

Targeted simultaneous C-to-T and A-to-G in rice protoplasts

Depositor

Caixia Gao

Insert

APOBEC3A-wtTadA-TadA7.10-nCas9-2X(T2A-UGI-NLS)

Expression

Plant

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJT176_GalL_eA3A-BE3

Plasmid

#145125

Purpose

Expressing base editor eA3A-BE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(N57G)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(N57G); spCas9(D10A)

Promoter

GalL

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT174_GalL_A3A-Y130F-BE3

Plasmid

#145123

Purpose

Expressing base editor A3A-Y130F-BE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(Y130F)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(Y130F); spCas9(D10A)

Promoter

GalL

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT173_GalL_A3A-R128A-BE3

Plasmid

#145122

Purpose

Expressing base editor A3A-R128A-BE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(R128A)-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(R128A); spCas9(D10A)

Promoter

GalL

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT113_GalL_cCDA1-xBE3

Plasmid

#145079

Purpose

Expressing base editor cCDA1-xBE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-xBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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ARB287d

Plasmid

#124018

Purpose

Encodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-spacer_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB287e

Plasmid

#124019

Purpose

Encodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-spacermulticistronic_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0507

Plasmid

#123998

Purpose

Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pPGK1-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0508

Plasmid

#123999

Purpose

Encodes pCAG driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCAG-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0509

Plasmid

#124000

Purpose

Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pSV40-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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