We narrowed to 13,578 results for: sequence
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Plasmid#184952PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mTagBFP2
ExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PPIB-bio-His
Plasmid#53413PurposeExpresses full-length Peptidyl-prolyl cis-trans isomerase B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPPIB (PPIB Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
VRQR variant
Plasmid#138562PurposeExpresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSDDepositorInsertVRQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335Q, T1337RPromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PECAM1-bio-His
Plasmid#51624PurposeExpresses full-length Platelet endothelial cell adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPECAM1 (PECAM1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSIREN-RetroQ-shN
Plasmid#73665PurposepSIREN-RetroQ vector containing control sequence NDepositorInsertshRNA Neg Control
UseRetroviralAvailable SinceMarch 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
L309-myc-seipin-WT
Plasmid#64061Purposemyc-tagged murine seipin (wild type); lentiviral constructDepositorAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF2_P2A_Hygro_Barcode
Plasmid#120489PurposeBarcoded lentiviral vector to express KLF2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
PM2-FRB-CFP
Plasmid#67518PurposeFRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)DepositorInsertmTOR (MTOR Human)
TagsCFP, HA, and PM tethering sequence from human Lyn…ExpressionMammalianMutationFRB domain only (residues 2019–2114)PromoterCMVAvailable SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)
Plasmid#197893PurposeCan be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertmCherry
UseAAVMutationN/APromoterEF1aAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF16_P2A_Hygro_Barcode
Plasmid#120502PurposeBarcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)
Plasmid#193854PurposePromoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing padDepositorInsert5' Cbr-unc-119(+) + 5' mNeonGreen
UseCRISPR and Cre/LoxExpressionWormPromoterCbr-unc-119pAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRNP-bio-His
Plasmid#51811PurposeExpresses full-length Major prion protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPRNP (PRNP Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MEF2C_P2A_Hygro_Barcode
Plasmid#120458PurposeBarcoded lentiviral vector to express MEF2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-1-333
Plasmid#104194PurposeExpresses human AR aa 1-333 in pGEX-5x-1 bacteral expression vector with GSTDepositorAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF3_P2A_Hygro_Barcode
Plasmid#120490PurposeBarcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
F2-bio-His
Plasmid#52179PurposeExpresses full-length Prothrombin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertF2 (F2 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-RTP2-Integration
Plasmid#129459PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses RTP2DepositorAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irC6kwh
Plasmid#44725DepositorInsertspCMV-D2i promoter
mCherry
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
RBM4_2xRRM-ZNF_pGEX
Plasmid#135154PurposeRecombinant expression of RBM4 with dual-affinity tagDepositorAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_hp53_ST
Plasmid#34922DepositorInsertp53 DNA-binding (Res 1-393) (TP53 Human)
ExpressionBacterialMutationC135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …PromoterT7Available SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only