We narrowed to 39,201 results for: ANT

1. pUTKan-3xHA-GFP-TOM5

   Plasmid

   #130674

   Purpose

   Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

   Depositor

   Andreas Weber

   Article

   Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

   Insert

   Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
   

   Use

   Tags

   3xHA and sGFP

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin10

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_B2203

   Plasmid

   #91066

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ-SpRY-Act3.0

   Plasmid

   #178957

   Purpose

   It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

   Plasmid

   #110555

   Purpose

   pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

   Depositor

   Martin Pule

   Article

   Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

   Insert

   Anti-RD114 heavy chain
   

   Use

   Tags

   mIgG2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1 alpha core promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_B2103b

   Plasmid

   #91062

   Purpose

   Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cathepsin S S21C/C25A

   Plasmid

   #84695

   Purpose

   for expression of mutant Cathepsin S in Leishmania tarantolae

   Depositor

   Hans Brandstetter

   Article

   Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

   Insert

   cathepsin S (CTSS Human)
   

   Use

   Expression in leishmania tarantolae

   Tags

   his-tag

   Expression

   Mutation

   S21C, C25A

   Promoter

   unknown

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJY-RpABE-PDS3_IMS

   Plasmid

   #112875

   Purpose

   binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

   Depositor

   Jae-Young Yun, Jin-Soo Kim

   Article

   Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

   Inserts

   RPS5A promoter
   ABE7.10
   U6-sgRNA targeting PDS3
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211845

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   6xUAS-35Smini and pUBQ10

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

   Plasmid

   #136382

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D570A

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-Flag CASTOR1 I280A

    Plasmid

    #84495

    Purpose

    overexpression of CASTOR1 I280A mutant

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 I280A (GATSL3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I280A

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pMOD_B2312

    Plasmid

    #91070

    Purpose

    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    PvUbi1

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_C2616

    Plasmid

    #91090

    Purpose

    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    At7SL

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_B2515b

    Plasmid

    #91073

    Purpose

    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOD_B2301

    Plasmid

    #91067

    Purpose

    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD_C2517

    Plasmid

    #91086

    Purpose

    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. iRFP713-N2-Frankenbody-Flag

    Plasmid

    #175291

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with iRFP713
    

    Use

    Tags

    iRFP713

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pMOD_B2615

    Plasmid

    #91078

    Purpose

    Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMpGWB122

    Plasmid

    #68576

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ266

    Plasmid

    #164713

    Purpose

    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    zCas9(D10A)-PmCDA1-UGI
    

    Use

    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-His-hPKM2

    Plasmid

    #42515

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pYPQ166-iSpyMac

    Plasmid

    #149553

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    iSpyMacCas9
    

    Use

    CRISPR; Gateway compatible zispymaccas9 entry clo…

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    R221K, N394K, Protospacer interacting domain form…

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pYPQ-dzCas9-NG-Act3.0

    Plasmid

    #158415

    Purpose

    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMOD_B2309

    Plasmid

    #91069

    Purpose

    Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    YAO

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSB161 - pL2_pSB90_2x35S::GUS::tMAS

    Plasmid

    #123197

    Purpose

    binary plant vector for transient expression of GUS (with introns)

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::GUS::tMAS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. SF-U7snRNA-CypA-E4

    Plasmid

    #190695

    Purpose

    Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

    Depositor

    Pim Pijnappel

    Article

    van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

    Insert

    U7 snRNA antisense CypA Exon 4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U7 promoter

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pYPQ-dAaCas12b-Act3.0

    Plasmid

    #158413

    Purpose

    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET30a-SARS-CoV2-ORF8

    Plasmid

    #165089

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pYPQ269C2

    Plasmid

    #164716

    Purpose

    Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    hAID-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible haid-cbe_v04 entry clo…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ269A2

    Plasmid

    #164714

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
    

    Use

    CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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