We narrowed to 5,173 results for: pcDNA3.1+

1. pcDNA_DiCre_59.F2

   Plasmid

   #60207

   Purpose

   Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

   Depositor

   Jean-Paul Herman

   Article

   Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

   Insert

   Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)
   

   Use

   Tags

   FKBP12 and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   amino acids 19-59 of the humanized version of Cre…

   Promoter

   CMV

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mc2 neo

   Plasmid

   #206214

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-HA-ERK2 Y261A

   Plasmid

   #8977

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y261A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pVH72-Tier1-PhCMV-SEAP

   Plasmid

   #169560

   Purpose

   Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven human secreted placental alkaline phosphatase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CENP-B mCherry PP1 gamma

   Plasmid

   #45221

   Depositor

   Iain Cheeseman, Michael Lampson

   Article

   Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

   Insert

   CENP-B mCherry PP1 gamma
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP2-NbV5

   Plasmid

   #201478

   Purpose

   BRET2 detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   GFP2-NbV5
   

   Use

   Tags

   GFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cenp-B DBD INCENP mCherry

   Plasmid

   #45233

   Purpose

   Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B-DBD INCENP-Δcen mCherry
   

   Use

   Tags

   DNA binding domain of CENP-B and mCherry

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-GFP-BIRC3-SU

   Plasmid

   #160699

   Purpose

   Expresses GFP-BIRC3 with the short 3'UTR in mammalian cells

   Depositor

   Christine Mayr

   Article

   Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

   Insert

   GFP-BIRC3 (BIRC3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. aq-CFP

   Plasmid

   #36073

   Depositor

   Narasimhan Gautam

   Article

   Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

   Insert

   G protein subunit alpha q (Gnaq Mouse)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   CFP inserted between amino acid 124 and 125

   Promoter

   CMV

   Available since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pGas-miRFP670nano3

    Plasmid

    #184672

    Purpose

    G protein alpha-subunit internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    G protein alpha-s-miRFP670nano3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Cav1-mRed

    Plasmid

    #12681

    Depositor

    Richard Pagano

    Article

    Sharma et al Mol Biol Cell. 2004 Jul . 15(7):3114-22.

    Insert

    canine caveolin-1 (CAV1 Canis familiaris)
    

    Use

    Tags

    mRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-GFP-BIRC3-LU

    Plasmid

    #160700

    Purpose

    Expresses GFP-BIRC3 with the long 3'UTR in mammalian cells

    Depositor

    Christine Mayr

    Article

    Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

    Insert

    GFP-BIRC3 (BIRC3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-FLAG-Fos AA

    Plasmid

    #8967

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362A, S374A

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-3xflagGREB1b

    Plasmid

    #112222

    Purpose

    mammalian expression of flag tagged GREB1b

    Depositor

    Craig Burd

    Article

    Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

    Insert

    GREB1b (GREB1 Human)
    

    Use

    Tags

    3x flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Septin 11 isoform IV

    Plasmid

    #120320

    Purpose

    Septin 11 isoform IV mammalian expression

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Septin 11 isoform I

    Plasmid

    #120314

    Purpose

    Septin 11 isoform I mammalian expression

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 2xFRB-myc

    Plasmid

    #20227

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. C-term Hec1-targeted Aurora B FRET sensor

    Plasmid

    #45222

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    C-term Hec1-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA PRAS40 S183A

    Plasmid

    #15674

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 S183A (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S183A

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

20. LUC56 (Luc/UGA/SP)

    Plasmid

    #112147

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cysteine 258mutated to selenocysteine (U)

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. h-alpha-ENaC-2

    Plasmid

    #83431

    Purpose

    full length human alpha ENaC-2 with flag tag

    Depositor

    Christie Thomas

    Article

    Thomas et al Am J Physiol. 1998 May;274(5 Pt 1):C1312

    Insert

    human alpha ENaC isoform 2 (SCNN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDWUER

    Plasmid

    #37094

    Depositor

    David Wang

    Article

    Gaynor et al PLoS Pathog. 2007 May 4. 3(5):e64.

    Insert

    WU polyomavirus early region (WUPyV_gp4, WUPyV_gp5 WU polyomavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YFP Gamma 1

    Plasmid

    #36101

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 1 (GNGT1 Bovine)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

24. vector-dendra2 PCDNA

    Plasmid

    #121134

    Purpose

    mammalian cell expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    empty cassete for contol of miR expression
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-IP3KC

    Plasmid

    #32527

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2000 Dec 1;352 Pt 2:343-51.

    Insert

    IP3KC (ITPKC Human)
    

    Use

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA-NLS-ZapCmR1.1

    Plasmid

    #59011

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-hBnvy

    Plasmid

    #18969

    Depositor

    Mariano Viapiano

    Article

    Hu et al J Biol Chem. 2008 Jul 7. ():.

    Insert

    Brevican uncleavable (BCAN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation at the ADAMTS cleavage site ESE/SRG: S…

    Promoter

    Available since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pTS2374-Tier1-PhCMV-Gal4_VP16

    Plasmid

    #169582

    Purpose

    Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    GAL4-transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA-SCW

    Plasmid

    #190633

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HA PRAS40 F129A

    Plasmid

    #15673

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 F129A (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F129A

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only