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We narrowed to 13,947 results for: CRISPR-Cas9

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Showing: 4601 - 4640 of 13947 results

  3. pCY5h

    Plasmid
    #160295
    Purpose
    Yeast CRISPR plasmid targeting the hphMX cassette
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160295-plasmid-map-sequence-id-464605

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  4. pCY5n

    Plasmid
    #160296
    Purpose
    Yeast CRISPR plasmid targeting the natMX cassette
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160296-plasmid-map-sequence-id-464604

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  5. pCY5k

    Plasmid
    #160294
    Purpose
    Yeast CRISPR plasmid targeting the kanMX cassette
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160294-plasmid-map-sequence-id-464601

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  6. pTX203

    Plasmid
    #89267
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsDEP1-gRNA02
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Promoter
    Maize Ubiquitin1 promoter
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89267-plasmid-map-sequence-id-177894

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  7. pTX202

    Plasmid
    #89266
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA01
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsDEP1-gRNA01
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Promoter
    Maize Ubiquitin1 promoter
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89266-plasmid-map-sequence-id-177842

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  16. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Promoter
    Tight TRE promoter
    Available Since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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  18. pDIV515

    Plasmid
    #177703
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177703-plasmid-map-sequence-id-353317

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  19. pDIV537

    Plasmid
    #177704
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    tRNA-sgRNA-tRNA
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177704-plasmid-map-sequence-id-352590

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  20. pDIV494

    Plasmid
    #177701
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177701-plasmid-map-sequence-id-352589

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  23. pDIV495

    Plasmid
    #177702
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Promoter
    RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177702-plasmid-map-sequence-id-353319

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  26. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  35. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  36. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  39. pX330 Mouse 5' Npm1 gRNA

    Plasmid
    #127900
    Purpose
    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127900-plasmid-map-sequence-id-249686

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  40. pX330 Mouse 3' Hp1a gRNA

    Plasmid
    #127898
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene
    Depositor
    Mark Groudine
    Insert
    gRNA/Cas9
    Use
    CRISPR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127898-plasmid-map-sequence-id-251076

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Showing: 4601 - 4640 of 13947 results