We narrowed to 5,273 results for: pcdna3.1
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pcDNA-Cx43-YA/VD
Plasmid#60699Purposemammalian expression of rat Cx43 YA/VD mutantDepositorInsertCx43 (Gja1 Rat)
UseTagsExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP-GIV-CT WT
Plasmid#69532PurposeMammalian expression of The CFP-tagged GIV-CT biosensorsDepositorInsertGIV (CCDC88A Human)
UseTagsCFPExpressionMammalianMutationC terminal amino acids S1660-S1870 of human GIVPromoterCMVAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTE4565
Plasmid#88903PurposeExpresses inactive/dead, humanized MbCpf1 nucleaseDepositorInserthMbCpf1(D986A)
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED31
Plasmid#15427DepositorInsertMED31 (Med31 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR182
Plasmid#106061PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR182; Opto-GPR182; F3)DepositorInsertOpto-GPR182 (GPR182 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR17
Plasmid#106005PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR17; Opto-GPR17; A7)DepositorInsertOpto-GPR17 (GPR17 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-HA-DNM2-W525L
Plasmid#155263PurposeMammalian expression of rat DNM2 fused to HADepositorInsertdynamin 2 (Dnm2 Rat)
UseTagsHAExpressionMammalianMutationTryptophan 525 to LeucinePromoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR37L1
Plasmid#106020PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37L1; Opto-GPR37L1; B10)DepositorInsertOpto-GPR37L1 (GPR37L1 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTE4567
Plasmid#107557PurposeExpresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.DepositorInserthMbCpf1(dead2)
UseTags3xHA and NLSExpressionMammalianMutationE1080APromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA FLAG mTOR ST
Plasmid#22999DepositorInsertmTOR (MTOR Human)
UseTagsFLAGExpressionMammalianMutationS2035TPromoterAvailable sinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR19
Plasmid#106007PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)DepositorInsertOpto-GPR19 (GPR19 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-human-BNIP3 delta Exon2
Plasmid#100782PurposeMammalian expression of BNIP3 delta Exon2 splice variantDepositorInserthuman-BNIP3 delta Exon2 (BNIP3 Human)
UseTagsHAExpressionMammalianMutationExon2 deletionPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRD397
Plasmid#163104PurposeExpression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mTurquoise2_H-NSdbd_NLS
UseEukaryotic expressionTagsExpressionMammalianMutationPromoterCMV promoter; T7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CNC3G (CASANOVA-C3 with glycine linkers)
Plasmid#137192PurposeExpresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory proteinDepositorInsertCNC3G (CASANOVA-C3 with glycine linkers)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIDx-ID-1058
Plasmid#135347PurposeExpression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminusDepositorInsertnSpyCas9-hAIDx-ID-1058-UGI
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPK-351
Plasmid#157921PurposepcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBDDepositorInsertsPIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD
UseSynthetic Biology; OptogeneticsTagsHA tag and SV40NLSExpressionMammalianMutationpif3 1-523PromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR75
Plasmid#106032PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR75; Opto-GPR75; C10)DepositorInsertOpto-GPR75 (GPR75 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEMAX-NLS-P2A_BLAST
Plasmid#135355PurposeExpression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertABEMax-2XNLS
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
RF-118-UB-BRCA1
Plasmid#78960PurposeExpression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertBRCA1 (BRCA1 Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-1155PromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR61
Plasmid#106027PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR61; Opto-GPR61; C5)DepositorInsertOpto-GPR61 (GPR61 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-EOMESwt-IDR
Plasmid#145231PurposeExpresses fusion of GAL4 DNA-binding domain and EOMESwt IDRDepositorInsertEOMESwt-IDR (EOMES Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-BHLHE41wt-IDR
Plasmid#145229PurposeExpresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDRDepositorInsertBHLHE41wt-IDR (BHLHE41 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXA13wt-IDR
Plasmid#145240PurposeExpresses fusion of GAL4 DNA-binding domain and HOXA13wt IDRDepositorInsertHOXA13wt-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXA13-58A-IDR
Plasmid#145238PurposeExpresses fusion of GAL4 DNA-binding domain and HOXA13-58A IDRDepositorInsertHOXA13-58A-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTE3320
Plasmid#107529PurposeExpresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.DepositorInserthLbCpf1(RVR)
UseTags3xHA and NLSExpressionMammalianMutationG532R, K538V, Y542RPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR88
Plasmid#106039PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR88; Opto-GPR88; D5)DepositorInsertOpto-GPR88 (GPR88 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA/Bmal L95E/L150E/W427A
Plasmid#47352PurposePcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003DepositorInsertBMAL (Bmal1 Mouse)
UseTagsHaExpressionMammalianMutationL95E/L150E/W427APromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_altPKA-Cα-mPAGFP
Plasmid#114133PurposeAlternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertTestis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR119
Plasmid#106040PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR119; Opto-GPR119; D6)DepositorInsertOpto-GPR119 (GPR119 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3m6L2
Plasmid#109366PurposeHuman rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tagDepositorInsertUseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTBL649 CHYRON2 integration construct
Plasmid#126446PurposeTo integrate the CHYRON2 locus at AAVS1 in human cells.DepositorInsertspU6-CHYRON2 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR135
Plasmid#106043PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR135; Opto-GPR135; D9)DepositorInsertOpto-GPR135 (GPR135 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR21
Plasmid#106009PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR21; Opto-GPR21; A11)DepositorInsertOpto-GPR21 (GPR21 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR1
Plasmid#105999PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR1; Opto-GPR1; A1)DepositorInsertOpto-GPR1 (GPR1 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-zeo: alpha6mGFP
Plasmid#35611Purposemammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tagDepositorInsertnAChr alpha6mEGFP (Chrna6 Mouse)
UseTagsmEGFP inserted after A405 with AGA linker on eith…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-Cα-mPAGFP
Plasmid#114131PurposeMammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertof the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cx43-YA/VD-Myc
Plasmid#60701Purposemammalian expression of Myc tagged rat Cx43 YA/VD mutantDepositorInsertCx43 (Gja1 Rat)
UseTagsMycExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.BRWD3-V5H
Plasmid#48624PurposeExpresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cellsDepositorInsertBRWD3 (BRWD3 Fly)
UseTagsV5 and His tagsExpressionMammalianMutationno mutations, stop was removed to fuse V5His at C…PromoterCMVAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only
