We narrowed to 7,677 results for: Trac

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pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Plasmid

#159872

Purpose

Three-fragment Gateway compatible protein tag for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTA-IRES-Hygro

Plasmid

#102423

Purpose

Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

tetracyclin-transactivator (rtTA)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-Munc18-1wt

Plasmid

#135550

Purpose

Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18-1wt

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209661

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

GFAP

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GRK2 S670D

Plasmid

#45756

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 S670D (GRK2 Bovine)

Expression

Mammalian

Mutation

S670D

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Myc-ASC

Plasmid

#73952

Purpose

Express c-Myc-tagged human ASC in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSEM272 - MCS mosTI Pmlc-2_GFP target

Plasmid

#159834

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI Pmlc-2_GFP target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA4b

Plasmid

#47589

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA4x/b (ATP2B4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K442R and P673L relative to NP_001675.3

Promoter

CMV

Available Since

Aug. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSin Helper

Plasmid

#127694

Purpose

Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Glycoproteins

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209659

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

GFAP

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

Plasmid

#182352

Purpose

Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSRE SSG

Plasmid

#127692

Purpose

Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Structural Genome

Expression

Mammalian

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Red Glifon Nega

Plasmid

#163117

Purpose

Red fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cells

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon Nega

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSin Capsid

Plasmid

#127693

Purpose

Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Capsid

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Expression

Bacterial

Promoter

AprR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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P55.SRE.mCMV.flag.mSrtA-3M.wpre

Plasmid

#226774

Purpose

secretory SortaseA in p53 (SRE backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD2

Plasmid

#20184

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 2 intracellular domain (Notch2 Mouse)

Tags

3X FLAG

Expression

Mammalian

Mutation

Contains murine Notch 2 Intracellular Domain (Met…

Promoter

CMV

Available Since

Jan. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

Plasmid

#182350

Purpose

Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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