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Showing: 4641 - 4680 of 6325 results

  5. CMV-mito-GEM-GECO1

    Plasmid
    #32461
    Purpose
    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO
    Use
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32461-plasmid-map-sequence-id-123705

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  7. piRFP670-N1-GalT

    Plasmid
    #87325
    Purpose
    To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87325-plasmid-map-sequence-id-177296

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  10. Gene Trap - 24XMS2 Lentiviral Vector

    Plasmid
    #174198
    Purpose
    Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.
    Depositor
    Daniel Larson
    Article
    Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.
    Insert
    iRFP
    Use
    Lentiviral
    Tags
    24X MS2 hairpins, Blasticidin, and T2A
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    174198-plasmid-map-sequence-id-371140

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  18. ATG-2460

    Plasmid
    #108713
    Purpose
    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt-copGFP fusion
    Use
    Lentiviral
    Tags
    CBR2opt fused to copGFP
    Expression
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    elongation factor-1
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108713-plasmid-map-sequence-id-224540

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  20. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

    Plasmid
    #209298
    Purpose
    A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.
    Depositor
    Birgitt Schuele
    Article
    Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1
    Inserts
    dCas9
    tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-inducible promoter (TRE3G)
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    209298-plasmid-map-sequence-id-414164

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  27. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid
    #169247
    Purpose
    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    RiboL1-jGCaMP8s
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169247-plasmid-map-sequence-id-343245

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  36. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid
    #128604
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Aug. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    128604-plasmid-map-sequence-id-457471

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  39. GABA(A) receptor subunit g2SE

    Plasmid
    #49170
    Purpose
    pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
    Use
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49170-plasmid-map-sequence-id-165320

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Showing: 4641 - 4680 of 6325 results