We narrowed to 18,742 results for: REV

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6xHis-MBP-BrCas12b

Plasmid

#182276

Purpose

Expresses recombinant BrCas12b

Depositor

Piyush Jain

Article

Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis, T7, Thrombin site

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMV361_MjTyrRS

Plasmid

#193250

Purpose

Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

Depositor

Peter Schultz, Jessica Seeliger

Article

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Insert

MjTyrRS

Expression

Bacterial

Promoter

hsp60

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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xlox dNGFR-TERT

Plasmid

#69805

Purpose

MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

Depositor

David Ott

Article

Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

Insert

hTERT reverse transcriptase (TERT Human)

Use

Retroviral

Tags

none

Promoter

MLV promoter

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA

Plasmid

#104097

Purpose

Expresses TREAT-PK co-localization control in mammalian cells

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

Renilla luciferase

Tags

24xMS2 stem loops in 3'UTR and 24xPP7 stem l…

Expression

Mammalian

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-P2A-CIAR_pLenti_CMV_Puro

Plasmid

#86503

Purpose

Lenti plasmid for generating CIAR expressing stable cell lines

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

GFP-P2A-CIAR (SOS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pPlacPbuCas13b-gRNA-T-MS2

Plasmid

#184839

Purpose

Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119 and lac promoter

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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CIAR_pcDNA5/FRT/TO

Plasmid

#86502

Purpose

Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CIAR (SOS1 Human)

Use

Frt

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV (tet operator)

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1-M7A

Plasmid

#159167

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1-M7A

Tags

SV40 NLS

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCLASP_Crz1(19A)_CLASP_RGS2membrane

Plasmid

#133085

Purpose

Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

Crz1*-CLASP

Expression

Bacterial and Yeast

Promoter

pADH1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-hB90

Plasmid

#18968

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

Insert

Brevican C-terminus (BCAN Human)

Expression

Mammalian

Mutation

Human brevican signal peptide aa 1-23 followed by…

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pUDR211

Plasmid

#113870

Purpose

expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA3-HXT8 / sgRNA4-HXT14

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDR418

Plasmid

#113875

Purpose

expression of double Cas9 programming sgRNA11 targetting STL1

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

doble sgRNA11-STL1

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCA528 huPQBP1

Plasmid

#217449

Purpose

Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

PQBP1 (PQBP1 Human)

Expression

Bacterial

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA Q67H/N74D

Plasmid

#217442

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q67H,N74D

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

Plasmid

#225228

Purpose

Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (25Q)

Tags

HA Tag and c-myc Tag

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

Plasmid

#225229

Purpose

Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

Depositor

Urartu Seker

Article

Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

Inserts

Aga2
Htt (46Q)

Tags

HA Tag and c-myc Tag

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCfB8709

Plasmid

#175224

Purpose

Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

Depositor

Irina Borodina

Article

Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

Insert

YOL092W (CEN.PK strain)

Use

Mrna expression, injection into xenopus oocytes

Tags

PolyA

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]

Plasmid

#106466

Purpose

Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(S219A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Serine 219 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

Plasmid

#106467

Purpose

Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(H474A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Histidine 474 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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