We narrowed to 17,122 results for: URE

1. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC-Myc-TRFcT

   Plasmid

   #44573

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   chimeric TRF (TERF2, TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TRF2 where TRFH domain (aa42-241) has been replac…

   Promoter

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

3. sgRNA BRCA2 V2102I

   Plasmid

   #139322

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 V2102I using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 V2102I using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA BRCA2 E2772K

   Plasmid

   #139323

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 E2772K using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 E2772K using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSN-A112C-GpA

   Plasmid

   #191238

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
   

   Use

   Tags

   nuclease A from S. aureus fused to the TM domain …

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine

   Promoter

   T7 promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-HA-ERK5 K84R

   Plasmid

   #53171

   Purpose

   Expresses GST-HA tagged human ERK5 K84R from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK5 (MAPK7 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   K84R

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

7. sgRNA BRCA2 R2842C

   Plasmid

   #139324

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 R2842C using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Plasmid

   #101233

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant G582A/V583A/E584A/D585A/N588A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   G582A, V583A, E584A, D585A and N588A

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-Myc-TRF1iDDR

   Plasmid

   #44576

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   chimeric TRF (TERF2, TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TRF1 containing the iDDR domain of TRF2 (aa407-43…

   Promoter

   Available since

   May 31, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pBabe-Myc-TRFcH

    Plasmid

    #44574

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    chimeric TRF (TERF2, TERF1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    TRF2 Hinge domain (aa245-443) replaced by that of…

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only