19,631 results

1. pKP332 (Lenti-OSK1)

   Plasmid

   #21627

   Depositor

   Tim Townes

   Article

   Chang et al Stem Cells. 2009 May . 27(5):1042-9.

   Insert

   hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Porcine teschovirus-1, Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-SFFV-PYL1-sfGFP-Emerin

   Plasmid

   #121532

   Purpose

   expression of PYL-SfGFP-Emerin, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   Emerin (EMD Human)
   

   Use

   Lentiviral

   Tags

   PYL1-SFGFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVpuro-CMV-N-3xFLAG

   Plasmid

   #123223

   Purpose

   N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-INU-TEX264-FLAG

   Plasmid

   #128258

   Purpose

   Expresses TEX264 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWJ66

   Plasmid

   #46570

   Purpose

   Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9-w
   CRISPR array
   

   Use

   CRISPR

   Tags

   rpoZ

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLIN2-V5-APEX2

   Plasmid

   #170573

   Purpose

   A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

   Depositor

   James Olzmann

   Article

   Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

   Insert

   PLIN2 (Plin2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5-APEX2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. piggyBAC-H2B mCherry-XL (R125K)

   Plasmid

   #189926

   Purpose

   Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

   Insert

   mCherry-XL (R125K)
   

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   differs from mCherry by R125K, W143S, I161V, Q163…

   Promoter

   CMV7

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOS017: ratiometric pHluorin (cytosolic)

   Plasmid

   #163052

   Purpose

   ratiometric pHluorin (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   ratiometric pHluorin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PPRE-pNL1.3

    Plasmid

    #84394

    Purpose

    Secreted Luciferase reporter for PPARgamma activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

    Insert

    PPARG response element (PPARG Human)
    

    Tags

    secNluc

    Expression

    Mammalian

    Promoter

    PPRE

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMA3790

    Plasmid

    #70110

    Purpose

    mtDNA elimination/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    hUNG1 Y147A
    Neo
    

    Tags

    None, human OTC mitochondrial targeting sequence,…

    Expression

    Mammalian

    Mutation

    Y147A, aa's 1-77 removed

    Promoter

    CMV, EF1a, and SV40 and native

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p(+)MV-NSE-FlagP-M502-3p

    Plasmid

    #58748

    Purpose

    Expresses recombinant measles virus with Flag-P gene tagged si2 (siP) target sequence

    Depositor

    Branka Horvat

    Insert

    Measles virus antigenome
    

    Tags

    Flag

    Promoter

    T7

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB501

    Plasmid

    #74843

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-Flag-CtIP-T847E

    Plasmid

    #71110

    Purpose

    mammalian expession of Flag-CtlP T847E mutation

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T847E

    Promoter

    TRE promoter, Tet ON

    Available Since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pHR_TetO_CD19-P2A-mCherry

    Plasmid

    #133803

    Purpose

    repressible expression of CD19 and mCherry in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    putative ECD of H. sapiens CD19 (CD19 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid

    #42335

    Purpose

    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. TrkB Acceptor

    Plasmid

    #83953

    Purpose

    FRET acceptor for TrkB mEGFP

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Phospholipase C gamma 1 (Plcg1 Mouse)
    

    Tags

    mRFP

    Expression

    Mammalian

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. NG-ABEmax

    Plasmid

    #124163

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    TadA-TadA(evo)-Cas9-NG
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hIBA1a-GFP-miR124T

    Plasmid

    #214147

    Purpose

    AAV vector to restrict GFP expression in microglia.

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Has Service

    AAV5

    Insert

    GFP
    

    Use

    AAV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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