Addgene

We narrowed to 13,587 results for: nts

Showing: 3541 - 3570 of 13587 results

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pCMB-ACTLr

Plasmid

#61194

Purpose

Fluorescent reporter for F-actin LifeAct-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL50005

Plasmid

#117536

Purpose

Level0 Golden Gate part: c-terminal tag, YFP

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

c-term tag YFP

Use

Synthetic Biology

Tags

Expression

Mutation

G65S, L68V, A72S, Y203T, L231H

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAK005

Plasmid

#128218

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 5

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 5

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAK003

Plasmid

#128216

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 3

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 3

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAK004

Plasmid

#128217

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 4

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 4

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK006

Plasmid

#128219

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 6

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 6

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMB-MTUBr

Plasmid

#61197

Purpose

Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MAP-MBD

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SpSrtA

Plasmid

#64987

Purpose

Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

S. pyogenes sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-81

Promoter

T7 promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGEL636

Plasmid

#189830

Purpose

The T-DNA backbone of Cas12j2 for transcriptional activation in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

pZmUbi1

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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P195

Plasmid

#139716

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A''_p35S:AsNpga:tAtug7

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAK002

Plasmid

#128215

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 2

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 2

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ER-moxMaple3

Plasmid

#120885

Purpose

mammalian expression of ER localized moxMaple3

Depositor

Erik Snapp

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Insert

moxMaple3

Use

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTFr

Plasmid

#61192

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCO602

Plasmid

#139735

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnCPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

CPH

Use

Luciferase; Moclo level 0 component

Tags

Expression

Mutation

Promoter

none

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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P364

Plasmid

#139721

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A'_pSlH4:NnCPH:tSlH4

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

SlH4

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO2214

Plasmid

#141303

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mCerulean and mScarlet-I

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO1267

Plasmid

#141301

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

gLUC and redLUC

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO4214

Plasmid

#141314

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mCerulean and mScarlet-I

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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XE69 Xenopus Beta-catenin in GFP

Plasmid

#16839

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
ZE33 Prickle-1-GFPnopren-pCS2+

Plasmid

#17074

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mutation

Pk1GFPnopren deletes the C-terminal six amino aci…

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET15b I-SceI (Ctermhis -MH)

Plasmid

#195613

Purpose

Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

Depositor

Frederick Gimble

Article

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-ChR2-mCherry

Plasmid

#32646

Purpose

Rabies vector pSADΔG expressing ChR2-mCherry for optogenetic excitation

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

ChR2

Use

Rabies virus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pICSL70008

Plasmid

#50336

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-uniRapR-P-Vav2(DPZ)

Plasmid

#91875

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL70004

Plasmid

#50334

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTRANS_233

Plasmid

#91122

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_480/pcDNA3

Plasmid

#85205

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_480

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSfi-HspLacZ

Plasmid

#33351

Depositor

David Kingsley, Doug Mortlock

Article

Mortlock et al Genome Res. 2003 Sep;13(9):2069-81. Epub

Insert

Hsp68 promoter (Hspa1a Mouse)

Use

Mouse Targeting

Tags

LacZ

Expression

Mammalian

Mutation

Promoter

Hsp68 (Hspa1a)

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only
pTRANS_101

Plasmid

#91199

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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