We narrowed to 9,406 results for: Coli

1. pmMaple2-CAM

   Plasmid

   #101149

   Purpose

   Fluorescent protein for superresolution imaging

   Depositor

   Xiaowei Zhuang

   Article

   Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

   Insert

   E. coli codon optimized mMaple2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CHIKV-nsP3-c001

   Plasmid

   #173086

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   N/A
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   A1334-T1493, codon optimized

   Promoter

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Plas-gRNA-LEU2

   Plasmid

   #157656

   Purpose

   GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   GFP-gRNA
   

   Use

   Insert storage (replicative in e. coli)

   Tags

   GFP-gRNA

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p70rg

   Plasmid

   #17827

   Depositor

   Jay Keasling

   Article

   Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

   Insert

   E. coli optimized red flourescent protein (RFPec), GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pIA1405

   Plasmid

   #172404

   Purpose

   Expression of SARS-COV-2 Nsp12 D218A in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   D218A

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIA1420

   Plasmid

   #172406

   Purpose

   Expression of SARS-COV-2 Nsp12 R116A in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   R116A

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIA1421

   Plasmid

   #172407

   Purpose

   Expression of SARS-COV-2 Nsp12 K50A in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSecEffector

   Plasmid

   #172891

   Purpose

   Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   Bxb1
   TP901
   TEV Display cassette
   ProD promoter between anti aligned Bxb1 sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProD, nTETO, and pBAD /AraC

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-nGFPMNase

   Plasmid

   #187826

   Purpose

   express nGFPMNase protein in E coli

   Depositor

   Yikang Rong

   Article

   Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

   Insert

   nGFPMNASE
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promotor

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICH75111

    Plasmid

    #50327

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, beta-glucuronidase (E. coli) with two introns,
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBXPC3H-Syb37

    Plasmid

    #123627

    Purpose

    Expresses Syb37 in e. coli cells, with Arabinose induction and Ampicillin selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    Syb37
    

    Use

    Tags

    3C-His and PelB

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEXP5-NT/pLux LasI

    Plasmid

    #193628

    Purpose

    Inducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LasI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Lux promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTYF-1xSYN-tTA

    Plasmid

    #19980

    Depositor

    Sergey Kasparov

    Article

    Liu et al J Gene Med. 2008 May . 10(5):583-92.

    Insert

    SYN-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIA1402

    Plasmid

    #172403

    Purpose

    Expression of SARS-COV-2 Nsp12 Y129A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Y129A

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJT103

    Plasmid

    #25087

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    PompC-lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETDuet-PcPT-ximD-ximE

    Plasmid

    #172654

    Purpose

    Production of decursinol in E. coli

    Depositor

    Minjuan Xu

    Article

    Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

    Insert

    PcPT, XimD and XimE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFLIP38

    Plasmid

    #65616

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD-pnGFP-Ultra

    Plasmid

    #157923

    Purpose

    Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

    Depositor

    Huiwang Ai

    Article

    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

    Insert

    pnGFP-Ultra, a high performance peroxynitrite sensor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICSL80016

    Plasmid

    #50332

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS no stop codon, beta-glucuronidase (E. coli) with two introns
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFLIP43

    Plasmid

    #65618

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edAFPt9-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQE80_T5_P3-ehaA_LacI

    Plasmid

    #186315

    Purpose

    Plasmid for inducible E coli expression of the P3 helixCAM.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Insert

    P3-ehaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQE80_T5_AP4-ehaA_LacI

    Plasmid

    #186316

    Purpose

    Plasmid for inducible E coli expression of the AP4 helixCAM.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Insert

    AP4-ehaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pIA1408

    Plasmid

    #172405

    Purpose

    Expression of SARS-COV-2 Nsp12 D760A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D760A

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Plas-CRISPR_reporter

    Plasmid

    #157658

    Purpose

    CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    dCAS9-VP64, mCherry, KanMX
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    Expression

    Mutation

    N28D in mCherry- please see depositor comments be…

    Promoter

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pReg-ROTU4

    Plasmid

    #190669

    Purpose

    Expresses an evolved rotundine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    ROTU4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGfOMT1-GEN4

    Plasmid

    #190612

    Purpose

    Expresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    GfOMT-GEN4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pReg-GLAU4

    Plasmid

    #190667

    Purpose

    Expresses an evolved glaucine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    GLAU4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pReg-PAP4

    Plasmid

    #190668

    Purpose

    Expresses an evolved papaverine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    PAP4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pReg-NOS4

    Plasmid

    #190670

    Purpose

    Expresses an evolved noscapine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    NOS4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVY-3 sfGFP-tag3

    Plasmid

    #194618

    Purpose

    fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

    Insert

    sfGFP-K(GGSKKRKKR)3
    

    Use

    Tags

    KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFLIP37

    Plasmid

    #65505

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-Cit-attR1 and attR2-Cer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28b-MseLig1

    Plasmid

    #107071

    Purpose

    Expresses M. sedula DNA Ligase 1 in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    DNA ligase 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-G2K

    Plasmid

    #70169

    Purpose

    bacterial expression of G2K (d-glycerate 2-kinase)

    Depositor

    George Reed, Paul Sims

    Article

    et al Journal of Molecular Catalysis B: Enzyma

    Insert

    garK/G2K
    

    Use

    Tags

    6xHIS and V5

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMH-HT_RcCHAD

    Plasmid

    #127782

    Purpose

    Expresses RcCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    RcCHAD
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAtLCYbSK

    Plasmid

    #53588

    Purpose

    Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

    Insert

    lcyB (LYC Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MS2-ecDHFR

    Plasmid

    #65859

    Purpose

    Expresses RNA-binding protein MS2 with eDHFR fusion tag

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

    Inserts

    MS2
    E. coli Dihydrofolate Reductase (folA )
    

    Use

    Tags

    HA and NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pCDW115

    Plasmid

    #70062

    Purpose

    artificial transcription template "pause ablation"

    Depositor

    Jeff Gelles

    Article

    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

    Insert

    lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pGEX-4T1 mouse Tankyrase1 5ARC (171–961)

    Plasmid

    #132638

    Purpose

    expresses mouse Tankyrase1 5ARC (171–961) in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Protein Sci. 2018 Jun;27(6):1057-1067. d

    Insert

    Tankyrase1 (171–961)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRSETA_crE6

    Plasmid

    #172928

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUC19-P70a-Bottom-T

    Plasmid

    #87313

    Purpose

    Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Bottom" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTH07

    Plasmid

    #70063

    Purpose

    artificial transcription template "distal pause"

    Depositor

    Jeff Gelles

    Article

    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

    Insert

    lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

42. 36INS-J23100_GB

    Plasmid

    #78669

    Purpose

    36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. 36INS-J23100_FB

    Plasmid

    #78668

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. 36INS-J23100_EB

    Plasmid

    #78667

    Purpose

    36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. 36INS-J23100_AB

    Plasmid

    #78666

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHBFD1

    Plasmid

    #53565

    Purpose

    Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    hbfd1 cDNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. pRL1058

    Plasmid

    #70686

    Purpose

    Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

    Depositor

    Peter Wolk

    Article

    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

    Insert

    Transposon Tn5-1058
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET-28a-CylA

    Plasmid

    #48198

    Purpose

    Expresses CylA (fatty acyl-AMP ligase) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylA
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. T7-AglC-His6

    Plasmid

    #89726

    Purpose

    Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglC
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. T7-AglK-His

    Plasmid

    #89714

    Purpose

    Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglK
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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