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TMEM230-WT-DsRed(isoform 2)

Plasmid

#99563

Purpose

mammalian expression of TMEM230-dsRed (isoform 2)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Tags

DsRed

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLViN-iRFP670-α-cateninA+

Plasmid

#229703

Purpose

Lentiviral expression of iRFP670-alpha-cateninA+ in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

amino acids 670-673, RAIM--> GSGS

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

Plasmid

#217766

Purpose

Fluorescent reporter for ceramide (for bacterial expression and purification)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Use

Protein purification (6xhis tag, tev site, sumo3 …

Tags

sfGFP

Expression

Bacterial

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

T7 promoter

Available Since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TMEM230-WT-pEGFP(isoform 2)

Plasmid

#99562

Purpose

mammalian expression of TMEM230-GFP (isoform 2)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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A3Ai E72A-Cas9n-UGI-NLS

Plasmid

#109430

Purpose

Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)

Use

CRISPR

Expression

Mammalian

Mutation

Insertion of an L1 intron into the coding sequenc…

Promoter

CMV

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 1)

Plasmid

#99561

Purpose

mammalian expression of TMEM230-GFP (isoform 1)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

Plasmid

#113158

Purpose

An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV, CRISPR, and Cre/Lox

Expression

Mammalian

Promoter

mU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-IRES(isoform 2)

Plasmid

#99564

Purpose

mammalian expression of TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

Plasmid

#113162

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Tags

FLAG

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-kappa-HA-dL5-myc-ADRB2

Plasmid

#101253

Purpose

Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)

Depositor

Marcel Bruchez

Article

Perkins et al Biochemistry. 2018 Feb 6;57(5):861-871.

Insert

kappa-HA-dL5-myc-ADRB2 (ADRB2 Human, Budding Yeast)

Use

Retroviral

Tags

FAP-dL5 and HA epitope

Expression

Mammalian

Promoter

MMLV LTR

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NES-IRES-hrGFP

Plasmid

#107544

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NES (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

Plasmid

#113156

Purpose

An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat Rosa26

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC-hTRF1deltaBLM

Plasmid

#64165

Purpose

Retroviral vector expressing human TRF1 delta aa317-374 with N-terminal MYC tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

deleted amino acids aa317-374 of human TRF1

Promoter

CMV

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
TMEM230-WT-IRES(isoform 1)

Plasmid

#99558

Purpose

mammalian expression of TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX(cre)-OCaMP-WPRE

Plasmid

#229853

Purpose

pAAV vector for Cre-dependent OCaMP (orange calcium indicator) expression under the control of hSyn promoter

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Aug. 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE

Plasmid

#224936

Purpose

pAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoter

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

Ef1a

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLViN-iRFP670-α-cateninΔβH

Plasmid

#229706

Purpose

Lentiviral expression of iRFP670-alpha-catenin-delta-beta-H in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-CTNNA1(α-catenin CRISPR KO)

Plasmid

#229708

Purpose

Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-α-cateninV-

Plasmid

#229696

Purpose

Transient expression of GFP-alpha-cateninV- in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L344P

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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