We narrowed to 10,944 results for: NSI;

1. pEGFP-C1-mScarlet-AMPKα2

   Plasmid

   #192451

   Purpose

   mScarlet-tagged alpha2 isoform of AMPK catalytic subunit.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   mScarlet-AMPKalpha2 (Prkaa2 Rat)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRD411

   Plasmid

   #178196

   Purpose

   sRok overexpression

   Depositor

   Remus Dame

   Article

   Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

   Insert

   srok
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRD231

   Plasmid

   #178195

   Purpose

   Rok overexpression

   Depositor

   Remus Dame

   Article

   Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

   Insert

   rok
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CC0101 BFPmut-stop-Bdnf.t1 (pEAQ)

   Plasmid

   #191173

   Purpose

   An orthogonal trigger sequence used in plant experiments, derived from the 3' UTR of murine Bdnf

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   Bdnf.t1 (plant)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EK0209 CMV-TO-mCherry-SG(s70587)SG-EGFP (FLP-IN)

   Plasmid

   #191174

   Purpose

   Mammalian expression of EK0285 with CMV-tetO promoter instead of SFFV.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   CMV-TO:mCherry:s70587:EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EK0494 SFFV-mCherry-SGc(CFL1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191181

   Purpose

   Expression of CFL1 sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:CFL1.s1:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

   Plasmid

   #191161

   Purpose

   Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   EGFP.t2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EK0341 SFFV-mCherry-SG(Bdnf.s1L153)SG-EGFP (FLP-IN)

   Plasmid

   #191150

   Purpose

   Expression of a 153 bp long sensor for murine Bdnf 3' UTR in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:Bdnf.s1L153:EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EK0358 SFFV-mCherry-SG(Bdnf.s1L36)SG-EGFP (FLP-IN)

   Plasmid

   #191152

   Purpose

   Expression of a 36 bp long sensor for murine Bdnf 3' UTR in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:Bdnf.s1L36:EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EK0290 SFFV-mCherry-SG(s70587-AND-s17d9b)SG-EGFP (FLP-IN)

    Plasmid

    #191143

    Purpose

    Expression of Sensor for (1 AND 2) "EK0208 AND EK0211" in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587-AND-s17d9b:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)

    Plasmid

    #191147

    Purpose

    Expression of the sequence matching the 54 extra bases in EK0362 in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    Bdfn.t+54
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSNRW-Z3-Nluc

    Plasmid

    #174486

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z3-Nluc
    

    Use

    Tags

    6x Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCfB9341 (pgRNA_IX-1_NatMX)

    Plasmid

    #161593

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB9343 (pgRNA_XV-1_NatMX)

    Plasmid

    #161595

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB9358(VII-1_Markerfree_BackBone)

    Plasmid

    #161599

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfB9359(VIII-1_Markerfree_BackBone)

    Plasmid

    #161600

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB9361(XIII-1_Markerfree_BackBone)

    Plasmid

    #161602

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB9363(XVI-1_Markerfree_BackBone)

    Plasmid

    #161604

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNCS-Clover4-LUMABS-HA

    Plasmid

    #183486

    Purpose

    To express the Clover4-LUMABS-HA sensor for Avian Influenza Virus antibody detection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-HA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNCS-Clover4-LUMABS-DEN1

    Plasmid

    #183487

    Purpose

    To express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.

    Depositor

    Jun Chu, Zongwen Jin

    Article

    Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

    Insert

    Clover4-LUMABS-DEN1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTriEx4_Rap1

    Plasmid

    #184662

    Purpose

    Mammalian expression of mouse Rap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSF-1b-S19-T48C

    Plasmid

    #128597

    Purpose

    Expresses tagless S19-T48C mutant 30S ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S19 (rpsS E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T48C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSF-1b-S15-T79C

    Plasmid

    #133048

    Purpose

    Expresses tagless S15-T79C mutant ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    S15 E.coli ribosomal protein (rpsO E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T79C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSF-1b-S7-S83C/Δ157-178

    Plasmid

    #128592

    Purpose

    Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    S7 E.coli ribosomal protein (rpsG E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S83C, Δ157-178

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-HA.HA.HA.Mm.RAP1

    Plasmid

    #184646

    Purpose

    Mammalian expression of mouse Rap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAV-U6+27-Tornado-Squash-SAM-sensor 4-2

    Plasmid

    #177916

    Purpose

    Expresses Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-Squash-SAM-sensor 4-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMCL8

    Plasmid

    #176184

    Purpose

    gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMCL8_28bp

    Plasmid

    #176185

    Purpose

    plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMCL9

    Plasmid

    #176186

    Purpose

    2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMC8b-XI1up

    Plasmid

    #176166

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMC7-XI1dw

    Plasmid

    #176167

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XI-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMC8b-XI2up

    Plasmid

    #176168

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMC7-XI2dw

    Plasmid

    #176169

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XI-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMC8b-XII2up

    Plasmid

    #176174

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XII-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMC7-XII2dw

    Plasmid

    #176175

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site XII-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMC8b-X4up

    Plasmid

    #176164

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site X-4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMC7-X4dw

    Plasmid

    #176165

    Purpose

    yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    3' homology for integration site X-4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TetO::EGFP-hMeikin(8A mut)

    Plasmid

    #174707

    Purpose

    Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pThuAsol

    Plasmid

    #171675

    Purpose

    pThuAsol expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    SUMO-TEV-ThuAsol
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTub-alpha-1-mtGFP

    Plasmid

    #138981

    Purpose

    Neuron-specific expression of membrane targeted EGFP

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Santos et al Cell Rep. 2020 Jul 21;32(3):107907. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP (added membrane targeting domain from GAP43).

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge