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Showing: 4761 - 4800 of 8780 results

  13. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

    Plasmid
    #153441
    Purpose
    E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode B knock-out mutant
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 140 and methionine 144 were mutated to a…
    Promoter
    T7 promoter
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153441-plasmid-map-sequence-id-306441

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  14. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid
    #153440
    Purpose
    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode A knock-out mutant
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 191 and leucine 195 on XDocB were mutate…
    Promoter
    T7 promoter
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153440-plasmid-map-sequence-id-306440

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  15. pUC19-Q03JI6

    Plasmid
    #103123
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
    Use
    CRISPR; Cloning vector
    Tags
    SV40NLS
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103123-plasmid-map-sequence-id-201885

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  22. YARS_pNIC-CTB10H

    Plasmid
    #153059
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    YARS (YARS1 Human)
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153059-plasmid-map-sequence-id-304479

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  23. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid
    #177328
    Purpose
    Control vector for Flp-dependent DREADD constructs
    Depositor
    Ulrik Gether
    Insert
    mCherry
    Use
    AAV; Flp-frt
    Expression
    Mammalian
    Promoter
    Human Synapsin-1
    Available Since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177328-plasmid-map-sequence-id-349583

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  24. pDUAL SRBI (GFP)

    Plasmid
    #86979
    Purpose
    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    SCARB1 (SCARB1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV/hPGK
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86979-plasmid-map-sequence-id-173289

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  25. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  26. pKT-PDGFRa-IRES-Katushka

    Plasmid
    #133081
    Purpose
    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    PDGFRa (Pdgfra Mouse)
    Expression
    Mammalian
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    133081-plasmid-map-sequence-id-261534

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  27. WARS_pNIC-CTB10H

    Plasmid
    #153058
    Purpose
    Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    WARS (WARS1 Human)
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153058-plasmid-map-sequence-id-304478

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  28. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Cre/Lox
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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  29. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  30. katushka-FMDV-P2A-pTRIP

    Plasmid
    #163522
    Purpose
    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert
    Depositor
    Thomas Weber
    Insert
    stopless Katushka-P2A
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163522-plasmid-map-sequence-id-321575

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  31. pBHM2642

    Plasmid
    #229526
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229526-plasmid-map-sequence-id-459924

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  32. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Patrik Verstreken
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172296-plasmid-map-sequence-id-340322

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  33. pPK719

    Plasmid
    #38149
    Depositor
    Patricia Kuwabara
    Insert
    cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)
    Use
    Recombineering
    Expression
    Worm
    Promoter
    unc-119
    Available Since
    Nov. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    38149-plasmid-map-sequence-id-54827

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  34. pENTRY4_ Pi21

    Plasmid
    #126904
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126904-plasmid-map-sequence-id-249827

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  35. pGGG-TaBSS2-GUS(int)

    Plasmid
    #242017
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic GUS (with introns) driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    GUS_with_introns
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242017-plasmid-map-sequence-id-504531

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  36. pGGG-TaBSS1-GUS(int)

    Plasmid
    #242013
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic GUS (with introns) driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    GUS_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242013-plasmid-map-sequence-id-504513

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  37. pGGG-TaBSS1-TaSEP1B6(int)

    Plasmid
    #242012
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic SEP1-B6 driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242012-plasmid-map-sequence-id-504511

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  38. pGGG-TaBSS1-TaMOFB1(int)

    Plasmid
    #242011
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of cisgenic MOF-B1 driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242011-plasmid-map-sequence-id-504506

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  39. pGGG-TaBSS2-tdTom

    Plasmid
    #242018
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 2 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS2
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    242018-plasmid-map-sequence-id-492286

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  40. pGGG-TaBSS1-tdTom

    Plasmid
    #242014
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242014-plasmid-map-sequence-id-487879

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Showing: 4761 - 4800 of 8780 results