We narrowed to 8,515 results for: SET

1. pCfB8748

   Plasmid

   #126918

   Purpose

   Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtE
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT2SC

   Plasmid

   #59382

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB8788

   Plasmid

   #126922

   Purpose

   Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtI and HMG1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRC319

   Plasmid

   #61272

   Purpose

   Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKAd5f11pES-PmeI

   Plasmid

   #122698

   Purpose

   To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

   Depositor

   Zhuo-Zhuang Lu

   Article

   Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ptRNAfMet-A37

   Plasmid

   #196657

   Purpose

   Plasmid for overexpression of native initiator tRNA (A37)

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   tRNAfMet-A37 expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   metY native promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSkipFlox

   Plasmid

   #85797

   Purpose

   P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   split Cre recombinase
   

   Use

   Cre/Lox; P. falciparum expression

   Tags

   FKBP, FRB*

   Expression

   Mutation

   Promoter

   Pfcrt

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

   Plasmid

   #179027

   Purpose

   Parent vector for markerless yeast integration cassettes with untagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-LRF

   Plasmid

   #66936

   Purpose

   Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

   Depositor

   Alvaro Monteiro

   Article

   Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV-40

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCfB8786

    Plasmid

    #126920

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME-FlEx switch

    Plasmid

    #82406

    Purpose

    Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgDonor_lentiGuide

    Plasmid

    #184168

    Purpose

    LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Insert

    sgDonor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHMattR3-attR4-GFP

    Plasmid

    #196247

    Purpose

    pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAH25-SceI

    Plasmid

    #129389

    Purpose

    Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Chloramphenicol acetyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    CAT promoter

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAbaMCi-GFP-gfp_pJMP2768

    Plasmid

    #208889

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ChrII_ttTi5605_loxP_myo2_neoR

    Plasmid

    #194056

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ttTi5605
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ChrV_oxTi365_loxP_myo2_neoR

    Plasmid

    #194057

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    oxTi365
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAP72_MS2

    Plasmid

    #220623

    Purpose

    Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAGD236

    Plasmid

    #219497

    Purpose

    pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    sfGFP, Eco1 msr, Eco1 RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSCL.140

    Plasmid

    #184995

    Purpose

    Express Cas9 in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TetOn-3g

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMyc-CMV1

    Plasmid

    #53582

    Purpose

    mammalian expression vector with Myc tag

    Depositor

    Linda Yu

    Article

    Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-eSIBR

    Plasmid

    #71664

    Purpose

    EGFP coding region followed by enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) backbone

    Depositor

    Philip Washbourne

    Article

    Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

    Insert

    EGFP coding sequence followed by eSIBR cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pME-eSIBR

    Plasmid

    #71665

    Purpose

    intronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)

    Depositor

    Philip Washbourne

    Article

    Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

    Insert

    intronic eSIBR cassette
    

    Use

    RNAi; Multi-site gateway

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)

    Plasmid

    #167357

    Purpose

    Integrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1t

    Depositor

    Michael Jensen

    Article

    Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

    Insert

    TDH3p:his6-mKate2-LipPks1-IDP1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSTK120-mCMV-TK

    Plasmid

    #22054

    Depositor

    Maria Castro

    Article

    King et al J Virol. 2008 May . 82(9):4680-4.

    Insert

    mCMV-TK-pA expression cassette
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pATF4-donor

    Plasmid

    #113805

    Purpose

    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP

    Depositor

    Kevin White

    Insert

    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    rs140428747

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CasRX_Pac

    Plasmid

    #194803

    Purpose

    CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

    Depositor

    Alexander Paredez

    Article

    Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

    Insert

    CasRX
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYR11 (XII-5:TDH3p:his6-mKate2-MBP-IDP1t)

    Plasmid

    #167356

    Purpose

    Integrative plasmid for cassette TDH3p:his6-mKate2-MBP-IDP1t

    Depositor

    Michael Jensen

    Article

    Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

    Insert

    TDH3p:his6-mKate2-MBP-IDP1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOCC82

    Plasmid

    #118862

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid

    #67279

    Purpose

    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    

    Use

    Tags

    linked to FlpO through an P2A ribosomal skipping …

    Expression

    Mammalian

    Mutation

    Promoter

    pdx

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNCS Citrine

    Plasmid

    #91761

    Purpose

    Bacterial expression of a fluorescent protein, Citrine

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Insert

    Citrine
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDGB3_alpha1-AtUBQ10:GFP-T35S

    Plasmid

    #211836

    Purpose

    Plant transient expression cassette for GFP (control) under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    GFP control
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN10

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTC201B

    Plasmid

    #52193

    Purpose

    for creating reversible targeting construct

    Depositor

    Tian Chi

    Article

    Chaiyachati et al BMC Biol. 2012 Nov 30;10:96. doi: 10.118

    Insert

    promoter-trap cassette Neo-Ires-GFP
    

    Use

    Cre/Lox

    Tags

    Neo, GFP

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRE57I-GNAc

    Plasmid

    #170637

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    Mini-Tn6677, GNAc cassette, lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LT0

    Plasmid

    #210551

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT0::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LT1

    Plasmid

    #210552

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT1::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LT2

    Plasmid

    #210553

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT2::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDonorTp

    Plasmid

    #211788

    Purpose

    pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR

    Depositor

    Silvia Cardona

    Article

    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

    Insert

    dhfr
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. BASIC_SEVA_66.11

    Plasmid

    #194213

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pICSL70008

    Plasmid

    #50336

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. mUAVbla:tetR 5′

    Plasmid

    #149379

    Purpose

    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Insert

    tetA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJB01

    Plasmid

    #111957

    Purpose

    Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Insert

    RFP
    

    Use

    Tags

    HaloTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pY3-LYS-IV

    Plasmid

    #218119

    Purpose

    LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. lentiCRISPRv2-Blast-mU6

    Plasmid

    #206806

    Purpose

    pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

    Depositor

    Jolanda Van Leeuwen

    Article

    Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pICSL70004

    Plasmid

    #50334

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAC155-pCR8-sgExpression

    Plasmid

    #49045

    Purpose

    U6-driven sgRNA expressing cassette on a gateway donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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