We narrowed to 29,083 results for: PLE;

1. pJS288

   Plasmid

   #200714

   Purpose

   To express yeast Hxt1 hexose transporter in yeast

   Depositor

   Jens Boch

   Article

   Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

   Inserts

   PMA1 promoter (PMA1 Budding Yeast)
   Hxt1 (HXT1 Budding Yeast)
   ADH2 Ter (ADH2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   domesticated for MoClo cloning (removal of intern…

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FDIO-COX4-dAPEX2

   Plasmid

   #117178

   Purpose

   Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   COX4-dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmPB2-VSV

   Plasmid

   #68038

   Purpose

   Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian cells

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Plexin-B2 (Plxnb2 Mouse)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-MARS-p85(iSH2)

   Plasmid

   #205236

   Purpose

   Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

   Depositor

   Jeremy Baskin

   Inserts

   PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
   p85(iSH2) (Pik3r1 Mouse)
   

   Use

   Tags

   Nuclear Export Sequence and mCherry

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FDIO-LACTB-dAPEX2

   Plasmid

   #117181

   Purpose

   Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   LACTB-dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FDIO-SYP-HRP

   Plasmid

   #117187

   Purpose

   Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   SYP-HRP
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Halotag-6xHp

   Plasmid

   #214414

   Purpose

   Lipid droplet localization of 6xHp of Spastin fused to Halotag

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   6x Spastin aa 43-92 (SPAST Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Il12b 400bp pro-Luc

   Plasmid

   #20020

   Depositor

   Stephen Smale

   Article

   Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

   Insert

   Il12b promoter (Il12b Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTP261

   Plasmid

   #104166

   Purpose

   Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
   

   Use

   Tags

   14x Histidine tag and NEDD8 from Brachypodium dis…

   Expression

   Bacterial

   Mutation

   1x Cysteine located at bps 493-495 in full sequen…

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFIN-RK-GFP-mIRBP156-CHER-WPRE

    Plasmid

    #44351

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Inserts

    RK-GFP
    mIRBP156-CHER
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human rhodopsin kinase (pos 2554-2850) and mouse …

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1932

    Plasmid

    #79781

    Purpose

    High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple259
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple259 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEMS1937

    Plasmid

    #79782

    Purpose

    High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple260
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple260 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAAV-DIO-IGK-dAPEX2-KDEL

    Plasmid

    #117183

    Purpose

    Plasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    IGK-dAPEX2-KDEL
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Seattle_IPDA_control_1_001

    Plasmid

    #167347

    Purpose

    ddPCR gating control for droplets containing all 5 targets (2 in pol)

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol_gag_tat_env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Seattle_IPDA_control_14_004

    Plasmid

    #167350

    Purpose

    ddPCR gating control for droplets containing the env target

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Seattle_IPDA_control_32_007

    Plasmid

    #167353

    Purpose

    ddPCR gating control for droplets containing 5'pol or 3'pol targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Seattle_IPDA_control_17_005

    Plasmid

    #167351

    Purpose

    ddPCR gating control for droplets containing gat and tat targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    gat_tat
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVL1393-MAL-SYK

    Plasmid

    #157990

    Purpose

    Expresses SYK in insect cell (sf9)

    Depositor

    Byung Il Lee

    Article

    Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

    Insert

    Spleen tyrosine kinase (SYK Human)
    

    Use

    Tags

    Met-Ala-Leu and add Leu,Glu residues +His tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-FDIO-IGK-dAPEX2-KDEL

    Plasmid

    #117184

    Purpose

    Plasmid name in publication: pAAV-FDIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    IGK-dAPEX2-KDEL
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFIN-XOPS-tdTOM-MOPS-GFP

    Plasmid

    #44347

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Inserts

    XOPS-tdTOM
    MOPS-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Xenopus Opsin (XOPS) (pos 3131-4495) and mouse op…

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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