We narrowed to 10,761 results for: T7
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pNP1950
Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term MBP NikJC199U
Plasmid#174365PurposeExpression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Tags8xHis, MBP, and TEVExpressionBacterialMutationC199UPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
bZiPro
Plasmid#86846Purposeexpress NS2B-NS3 protease in E.coliDepositorInsertsNS3 1-177
NS2B45-96
Tagsa hexahistidine tag and a thrombin cleavage siteExpressionBacterialMutationNS2B cofactor region (residues 45–96) and NS3 pro…PromoterT7Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
Tags3xFlag and intein-CBDExpressionBacterialPromoterT7Available SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mClover3
Plasmid#74236PurposeConstitutive expression of mClover3 in bacteriaDepositorHas ServiceCloning Grade DNAInsertmClover3
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b-pknG
Plasmid#102814PurposeExpression of PknG in E.coliDepositorInsertpknG
TagsHisExpressionBacterialPromoterT7Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE1
Plasmid#73018PurposeExpresses BE1 with N-terminal His6 tag in E. ColiDepositorInsertBE1
TagsHis6 tagExpressionBacterialPromoterT7Available SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-NanoLuc
Plasmid#188247PurposeTemplate plasmid to transcribe mRNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseExpressionMammalianPromoterT7Available SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 pET15b
Plasmid#60678PurposeRecombinant protein expression of Park7DepositorAvailable SinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
SUMO_LPETG_MT3
Plasmid#200304PurposeBacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavageDepositorAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD-MSP1D1
Plasmid#161607PurposeBacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formationDepositorInsertD-MSP1D1
Tags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans were replaced b…PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
TagsMGHHHHHHGGAMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm4b-pA
Plasmid#104486PurposeExpress mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.DepositorAvailable SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MLM3613
Plasmid#42251PurposeExpresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promotersDepositorInsertStreptococcus pyogenes Cas9
UseCRISPR; Zebrafish expressionPromoterCMVAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1-Strep-II
Plasmid#224209PurposeMembrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.DepositorInsertMSP1D1-Strep-II
TagsStrep-II TagExpressionBacterialMutationSubstitution of 7xHis tag found in pMSP1D1 for a …PromoterT7Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP1SA11
Plasmid#89162PurposeExpress Rotavirus SA11 VP1 in mammalian cellsDepositorInsertRotavirus SA11 VP1
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2-sfGFP
Plasmid#88921PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
His6_BcLOV4_mCherry_BamUK
Plasmid#114596PurposeEncodes mCherry-tagged BcLOV4 for expression in bacterial cellsDepositorInsertBcLOV4
TagsHis6 and mCherryExpressionBacterialPromoterT7Available SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mRuby3
Plasmid#74234PurposeConstitutive expression of mRuby3 in bacteriaDepositorInsertmRuby3
Tags6xHisExpressionBacterialPromoterT7Available SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(CysCNP)RS
Plasmid#225054PurposetRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codonDepositorArticleInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7-NSP4SA11
Plasmid#89171PurposeExpress Rotavirus SA11 NSP4 in mammalian cellsDepositorInsertRotavirus SA11 NSP4
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP2SA11
Plasmid#89163PurposeExpress Rotavirus SA11 VP2 in mammalian cellsDepositorInsertRotavirus SA11 VP2
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-NSP3SA11
Plasmid#89170PurposeExpress Rotavirus SA11 NSP3 in mammalian cellsDepositorInsertRotavirus SA11 NSP3
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP4SA11
Plasmid#89165PurposeExpress Rotavirus SA11 VP4 in mammalian cellsDepositorInsertRotavirus SA11 VP4
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP3SA11
Plasmid#89164PurposeExpress Rotavirus SA11 VP3 in mammalian cellsDepositorInsertRotavirus SA11 VP3
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-NSP5SA11
Plasmid#89172PurposeExpress Rotavirus SA11 NSP5 in mammalian cellsDepositorInsertRotavirus SA11 NSP5
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-NSP2SA11
Plasmid#89169PurposeExpress Rotavirus SA11 NSP2 in mammalian cellsDepositorInsertRotavirus SA11 NSP2
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE80l ACA
Plasmid#196471PurposeProtein expression of SpyTag-ELP-CarHC-ELP-SpyTagDepositorInsertACA
ExpressionBacterialPromoterT7Available SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP6SA11
Plasmid#89166PurposeExpress Rotavirus SA11 VP6 in mammalian cellsDepositorInsertRotavirus SA11 VP6
ExpressionMammalianPromoterT7 promotorAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-PhoCl2c
Plasmid#164035PurposeImproved PhoCl variant with enhanced dissociation efficiencyDepositorInsertPhoCl2c
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet-MBP
Plasmid#209184PurposeExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coliDepositorTypeEmpty backboneTagsHisX6ExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP7SA11
Plasmid#89167PurposeExpress Rotavirus SA11 VP7 in mammalian cellsDepositorInsertRotavirus SA11 VP7
Tags*see belowExpressionMammalianPromoterT7 promotorAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-FLuc
Plasmid#188311PurposeExpression of Firefly luciferase via a T7 promoter in TXTLDepositorInsertFirefly luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialPromoterT7MaxAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only
