We narrowed to 4,881 results for: phy

1. beta-catenin (S33Y)-pcw107

   Plasmid

   #64615

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S33Y

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

   Plasmid

   #64613

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   S33A, S37A, T41A, S45A

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p53 (dominant negative R175H mutant)-pcw107-V5

   Plasmid

   #64638

   Purpose

   3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   TP53 (transcript variant 3) (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   R175H

   Promoter

   PGK

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SmoM2 (W535L)-pcw107

   Plasmid

   #64627

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   SMO (SMO Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   W535L

   Promoter

   PGK

   Available since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. myr-FLAG-AKT1-pcw107

   Plasmid

   #64606

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and myristoylated

   Expression

   Mutation

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Kras (G12V)-pcw107

   Plasmid

   #64602

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Notch1 intracellular domain-pcw107-V5

   Plasmid

   #64622

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   NOTCH1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   intracellular domain *

   Promoter

   PGK

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

8. beta-catenin (S33A, S37A, T41A, S45A)-pcw107

   Plasmid

   #64612

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S33A, S37A, T41A, S45A

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET His6 MBP TEV HDAC8_374

   Plasmid

   #122174

   Purpose

   Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

   Depositor

   David Christianson

   Article

   Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

   Insert

   His6 MBP TEV HDAC8_374 (HDAC8 Human)
   

   Use

   Tags

   hexahistidine tag and tobacco etch virus protease…

   Expression

   Bacterial

   Mutation

   Residues Met1–Pro7 and H375-V377 have been delete…

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AR-V7-pcw107

    Plasmid

    #64635

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    AR (transcript variant 1, splice isoform) (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    splice isoform*

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. beta-catenin (S33Y)-pcw107-V5

    Plasmid

    #64616

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S33Y

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

12. IKKalpha (S176E,S180E)-pcw107-V5

    Plasmid

    #64608

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S176E, S180E

    Promoter

    PGK

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AR-V7-pcw107-V5

    Plasmid

    #64636

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    AR (transcript variant 1, splice isoform) (AR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    splice isoform *

    Promoter

    PGK

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Notch1 intracellular domain-pcw107

    Plasmid

    #64621

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    NOTCH1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    intracellular domain *

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Hras (G12V)-pcw107

    Plasmid

    #64603

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    HRAS (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pSAD-5PSD95-EGFP-SynPhRFP

    Plasmid

    #217979

    Purpose

    G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

    Depositor

    Edward Callaway

    Article

    Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

    Inserts

    5PSD95-EGFP (Dlg4 Rat)
    SynPhRFP (Syp Mouse)
    

    Use

    G-deleted rabies

    Tags

    EGFP and RFP

    Expression

    Mutation

    Promoter

    None

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MEK5 DD(S311D,T315D)-pcw107-V5

    Plasmid

    #64619

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K5 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    S311D, T315D*

    Promoter

    PGK

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

18. p38 WT O/E (MAPK14)-pcw107-V5

    Plasmid

    #64624

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAPK14 (transcript variant 2) (MAPK14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID

    Plasmid

    #122981

    Purpose

    AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    Syph-GFP-myc-BoNT/B(1-146)-iLID (Syp Rat, Synthetic)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MEK1-pcw107-V5

    Plasmid

    #64650

    Purpose

    (control) when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

21. ERalpha (Y537S mutant)-pcw107-V5

    Plasmid

    #64634

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    ESR1 (transcript variant 1) (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Y537S

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

22. Stat3 (A662C,N664C,V667L)-pcw107-V5

    Plasmid

    #64611

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    A662C, N664C, V667L

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

23. Rgl2-CAAX-pcw107-V5

    Plasmid

    #64641

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Rgl2 plus C-term KRAS (Kras Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    C-term of Kras

    Promoter

    PGK

    Available since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

24. Gli2 truncation-pcw107-V5

    Plasmid

    #64626

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    GLI2 (truncation mutant) (GLI2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    truncated

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

25. Caspase-3 (C163A)-pcw107-V5

    Plasmid

    #64633

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    CASP3 (transcript variant beta) (CASP3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    C163A

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pBABE-2xFLAG-2xSTREP-CCND3-Puro

    Plasmid

    #172622

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBABE-2xFLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172628

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. JNK2 WT O/E (MAPK9)-pcw107-V5

    Plasmid

    #64617

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAPK9 (transcript variant JNK2-a2) (MAPK9 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

29. FLAG-MKK6 (S207E,T211E)-pcw107

    Plasmid

    #64625

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K6 (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    S207E,T211E

    Promoter

    PGK

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

30. myr-FLAG-MEK5-pcw107

    Plasmid

    #64620

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    MAP2K5 (MAP2K5 Human)
    

    Use

    Lentiviral

    Tags

    Flag and myristoylated

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

31. FLAG-YAP2 (8SA)-pcw107

    Plasmid

    #64637

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    YAP2 (transcript variant 3) (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    8SA*

    Promoter

    PGK

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

32. PX458-gR2A_ISO

    Plasmid

    #124808

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    hSPCas9
    

    Use

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBABE-2xFLAG-2xSTREP-CCND1(T286A)-Puro

    Plasmid

    #172633

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBABE-2xFLAG-2xSTREP-CCND1-Puro

    Plasmid

    #172634

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HRas (G12V, E37G)-pcw107

    Plasmid

    #64639

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    HRAS (transcript variant 3) (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G12V, E37G

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

36. HRas (G12V, E37G)-pcw107-V5

    Plasmid

    #64640

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    HRAS (transcript variant 3) (HRAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    G12V, E37G

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PX459-gR2A_Hinge

    Plasmid

    #124811

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Cas9 – gRNA_R2A_hinge
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    R166H in PuroR (please see depositors comment bel…

    Promoter

    pUC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Human SHIP2

    Plasmid

    #124645

    Purpose

    Expresses HIS tagged human SHIP2 in COS-7/HEK cells

    Depositor

    Christophe Erneux

    Article

    Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-5

    Insert

    INPPL1 (INPPL1 Human)
    

    Use

    Tags

    His Xpress (N-terminal)

    Expression

    Mammalian

    Mutation

    coding sequence starts at amino acid 18 as calcul…

    Promoter

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEMS1238

    Plasmid

    #29120

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple153 (OXT Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEMS1237

    Plasmid

    #29119

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple152 (OXT Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge