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We narrowed to 7,219 results for: cas9 plasmid

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Showing: 6051 - 6100 of 7219 results

  3. pLD-puro-Cc-CR-PARK7WT-VA

    Plasmid
    #115187
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115187-plasmid-map-sequence-id-225414

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  4. pLD-puro-Cc-CR-PARK7V51G-VA

    Plasmid
    #115188
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115188-plasmid-map-sequence-id-225424

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  5. pLD-puro-Cc-CR-PARK7C53A-VA

    Plasmid
    #115189
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115189-plasmid-map-sequence-id-225429

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  6. pLD-puro-Cc-CR-PARK7H126A-VA

    Plasmid
    #115190
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115190-plasmid-map-sequence-id-225431

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  7. pLD-puro-Cc-CR-PARK7E163K-VA

    Plasmid
    #115191
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
    Use
    Lentiviral
    Tags
    Versatile affinity tag (2XStreptactin II-6XHis-3X…
    Mutation
    c.150G>A (silent when translated to protein, c…
    Promoter
    CMV
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115191-plasmid-map-sequence-id-225432

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  8. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid
    #223125
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223125-plasmid-map-sequence-id-447703

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  13. pRBPJ.1.0-gDNA

    Plasmid
    #132474
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RBPJ (RBPJ Human)
    Use
    CRISPR
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132474-plasmid-map-sequence-id-267181

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  14. pAAV-BiSSTe4-dSaCa9-KRAB(Zim3)-pA

    Plasmid
    #255031
    Purpose
    AAV plasmid encoding a dSaCas9-KRAB(Zim3) fusion gene controlled from an SST-specific neuronal enhancer, BiSSTe4
    Depositor
    Jonathan Ploski
    Insert
    dSaCas9-KRAB(Zim3)
    Use
    AAV and CRISPR
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255031-plasmid-map-sequence-id-509446

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  16. pYTP MYRF sg1

    Plasmid
    #88857
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg1 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg1 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88857-plasmid-map-sequence-id-179037

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  17. pYTP MYRF sg2

    Plasmid
    #88858
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg2 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg2 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88858-plasmid-map-sequence-id-172479

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  18. pYTP MYRF sg3

    Plasmid
    #88859
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg3 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg3 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88859-plasmid-map-sequence-id-172480

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  19. pYTP MYRF sg4

    Plasmid
    #88860
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg4 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg4 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88860-plasmid-map-sequence-id-179756

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  20. pYTP MYRF sg5

    Plasmid
    #88861
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg5 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg5 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88861-plasmid-map-sequence-id-172482

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  21. pYTP MYRF sg6

    Plasmid
    #88862
    Purpose
    pAC154-dual-dCas9VP160-sgExpression with MYRF sg6 cloned into BbsI site
    Depositor
    Mahmoud Pouladi
    Insert
    dCas9VP160 and MYRF sg6 gRNA (MYRF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    88862-plasmid-map-sequence-id-172483

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  22. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid
    #242662
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242662-plasmid-map-sequence-id-489091

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  23. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid
    #242661
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242661-plasmid-map-sequence-id-489090

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  24. plenti-As-Cas12a-2xNLS

    Plasmid
    #155047
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    AsCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155047-plasmid-map-sequence-id-308054

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  25. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155046-plasmid-map-sequence-id-308670

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  26. B2M Bulldozer (gRNA crB2M_13)

    Plasmid
    #84381
    Purpose
    This plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cells
    Depositor
    Chad Cowan
    Article
    Mandal et al Cell Stem Cell. 2014 Nov 6;15(5):643-52.
    Insert
    B2M Bulldozer crB2M_13 gRNA
    Expression
    Mammalian
    Promoter
    hU6 promoter
    Available Since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84381-plasmid-map-sequence-id-189203

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  39. pORFE0002

    Plasmid
    #112071
    Purpose
    AtCAS9 nickase D10A module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 nickase D10A
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112071-plasmid-map-sequence-id-217629

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  40. pORFE0003

    Plasmid
    #112072
    Purpose
    AtDeadCAS9 D10A H840A module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112072-plasmid-map-sequence-id-217628

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  41. PLJR965_Rv3297

    Plasmid
    #241235
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv3297) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv3297 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241235-plasmid-map-sequence-id-483383

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  42. PLJR965_Rv2976c

    Plasmid
    #241234
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2976c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2976c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241234-plasmid-map-sequence-id-483384

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  43. PLJR965_Rv2464c

    Plasmid
    #241232
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2464c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2464c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241232-plasmid-map-sequence-id-483381

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  44. PLJR965_Rv1638

    Plasmid
    #241231
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1638) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1638 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241231-plasmid-map-sequence-id-483389

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  45. PLJR965_Rv1259

    Plasmid
    #241228
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1259) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1259 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241228-plasmid-map-sequence-id-483385

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  46. PLJR965_Rv1020

    Plasmid
    #241227
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1020) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1020 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241227-plasmid-map-sequence-id-483388

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  47. PLJR965_Rv0949

    Plasmid
    #241226
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv0949) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv0949 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241226-plasmid-map-sequence-id-483386

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  48. PLJR965_Rv1633

    Plasmid
    #241230
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv1633) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv1633 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241230-plasmid-map-sequence-id-483390

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  49. PLJR965_Rv3589

    Plasmid
    #241236
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv3589) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv3589 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241236-plasmid-map-sequence-id-483387

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  50. PLJR965_Rv2924c

    Plasmid
    #241233
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2924c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2924c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    241233-plasmid-map-sequence-id-483382

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Showing: 6051 - 6100 of 7219 results