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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,962 results for: tet on

Showing: 6051 - 6100 of 6962 results

  6. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Peter Schultz
    Article
    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    49086-plasmid-map-sequence-id-396431

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  8. PB-CAG::Nucbow

    Plasmid
    #158996
    Purpose
    Multicolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Nucbow
    Use
    Tags
    All FPs are fused to H2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158996-plasmid-map-sequence-id-311805

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  10. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

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  12. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Use
    Tags
    3x-FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    pTet
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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  20. AAV-tTA

    Plasmid
    #175280
    Purpose
    AAV vector mediating neuron-specific expression of tTA
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    tTA advanced (improved tetracycline-controlled transactivator)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175280-plasmid-map-sequence-id-349089

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  24. pCEP4-BaLgp140-TM

    Plasmid
    #111843
    Purpose
    Mammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tether
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (BaL) Env
    Use
    Tags
    CD5 leader sequence and TM helix from MHC class I
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111843-plasmid-map-sequence-id-210251

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  38. CMV+ episome

    Plasmid
    #169742
    Purpose
    CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169742-plasmid-map-sequence-id-334220

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  39. PGK+ episome

    Plasmid
    #170041
    Purpose
    PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    170041-plasmid-map-sequence-id-334558

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  40. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Use
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  41. pKL001

    Plasmid
    #98917
    Purpose
    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    CaPR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23118 - Strong Constitutive
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    98917-plasmid-map-sequence-id-189879

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  43. pNHEJ-Cas9-recX

    Plasmid
    #158715
    Purpose
    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    cas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158715-plasmid-map-sequence-id-321939

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  46. CMV+ plasmid

    Plasmid
    #169740
    Purpose
    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169740-plasmid-map-sequence-id-334212

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Showing: 6051 - 6100 of 6962 results