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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Article

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
RSV-Sp1

Plasmid

#12098

Depositor

Robert Tjian

Insert

Sp1 (SP1 Human)

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Plasmid

#38153

Depositor

Nora Heisterkamp

Article

Zhang et al Leukemia. 2007 Jun;21(6):1189-97. Epub 2

Inserts

N-cadherin (CDH2 Human)
EGFP

Use

Lentiviral

Promoter

MNDU3c = LTR promoter for U3 region of myeloproli…

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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-3.6kb human IFN-gamma luc

Plasmid

#17599

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CAPN2

Plasmid

#60942

Purpose

Expresses calpain2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain 2 (CAPN2 Human)

Tags

3xflag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Promoter

human synapsin

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-ASC

Plasmid

#73952

Purpose

Express c-Myc-tagged human ASC in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-Ago2

Plasmid

#21538

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

The first 7aa of this Ago2 protein are different …

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_AGIA-AirID

Plasmid

#182210

Purpose

Express AirID in mammalian cells.

Depositor

Sohei Ito, Shogo Nakano, Akira Nozawa, Tatsuya Sawasaki

Article

Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

Insert

AGIA-AirID

Tags

AGIA-tag

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCSDest

Plasmid

#22423

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Type

Empty backbone

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pmCherry-2xFYVE

Plasmid

#140050

Purpose

Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available Since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

Plasmid

#84469

Purpose

pAAV-RAM-EGFP

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

EGFP

Use

AAV

Promoter

TRE

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-LKB1

Plasmid

#21147

Depositor

Junying Yuan

Article

Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

Insert

GFP-LKB1 (Stk11 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
pTriEx-mCherry-PA-Rac1-T17N

Plasmid

#22029

Purpose

Expression of photoactivatable Rac1 (dominant-negative mutant).

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6

Plasmid

#11974

Purpose

For mammalian expression of human ATF6 with an HA tag

Depositor

Ron Prywes

Article

Zhu et al Mol Cell Biol. 1997 Sep . 17(9):4957-66.

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pECFP-DEVDR-Venus

Plasmid

#24537

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVDR-Venus

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

Plasmid

#61629

Purpose

R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

Depositor

Dolf Weijers

Article

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

mDII
ntdTomato
DII
n3xVenus

Expression

Plant

Promoter

RPS5A

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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p181 pK7-HDAC1 (GFP)

Plasmid

#11054

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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