We narrowed to 15,050 results for: pET

1. pET-28a RUVBL2 Full Length

   Plasmid

   #159142

   Purpose

   Expresssion and protein purification of full length RUVBL2

   Depositor

   Eric Erquan Zhang

   Article

   Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

   Insert

   RUVBL2 (RUVBL2 Human)
   

   Expression

   Bacterial

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. human EB1 in PET28a for protein expression

   Plasmid

   #39297

   Depositor

   Jennifer Tirnauer, Tim Mitchison

   Article

   Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   6HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pet151D-TOPO ALIX Bro1-V (1-702)

   Plasmid

   #80642

   Purpose

   Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648

   Depositor

   Wesley Sundquist

   Article

   McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

   Insert

   ALIX Bro1-V (PDCD6IP Human)
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Residues 1-702

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-CohIII-CBM-HIS-ybbR

   Plasmid

   #60866

   Purpose

   Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

   Depositor

   Hermann Gaub

   Article

   Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

   Inserts

   CohIII
   CBM
   

   Tags

   6xHIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   Cysteine 63 to Serine in CBM module

   Promoter

   T7

   Available Since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-E. coli Initiation Factor 3 (IF3)

   Plasmid

   #31340

   Depositor

   Linda Spremulli

   Article

   Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

   Insert

   E. coli Initiation Factor 3
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pET His6 MBP TEV HDAC8_374

   Plasmid

   #122174

   Purpose

   Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

   Depositor

   David Christianson

   Article

   Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

   Insert

   His6 MBP TEV HDAC8_374 (HDAC8 Human)
   

   Tags

   hexahistidine tag and tobacco etch virus protease…

   Expression

   Bacterial

   Mutation

   Residues Met1–Pro7 and H375-V377 have been delete…

   Promoter

   T7

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

   Plasmid

   #136684

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-His tagged nuclear Pif1

   Plasmid

   #65047

   Purpose

   expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

   Depositor

   Jean-Baptiste Boulé, Virgina Zakian

   Article

   Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

   Insert

   Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
   

   Tags

   histag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-HIS-Xyl-Xmod-DocIII

   Plasmid

   #60865

   Purpose

   Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

   Depositor

   Hermann Gaub

   Article

   Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

   Inserts

   Xyl
   Xmod-DocIII
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Threonine 120 to Cysteine in Xylanase domain

   Promoter

   T7

   Available Since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-28-Strep-OPA1(MGD) (SB264)

    Plasmid

    #227613

    Purpose

    Inducible expression of Strep-tagged Opa1(MGD) in E.coli

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Tags

    Twin-StrepTag

    Expression

    Bacterial

    Mutation

    contains aa 253-580 (MGD) and aa 938-960

    Promoter

    T7

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-(-30)GFP-9xGGS-Cre-6xHis

    Plasmid

    #62372

    Purpose

    Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP-9xGGS-Cre-6xHis
    

    Tags

    (-30)GFP and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-His6-Sumo-CfaC-MBP

    Plasmid

    #126018

    Purpose

    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-CfaC-MBP
    

    Tags

    6xHis-SUMO and HA-MBP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-mTagBFP2-TEV-Strep-His

    Plasmid

    #210824

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mTagBFP2
    

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-15b-hAR-663-919

    Plasmid

    #89083

    Purpose

    bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

    Insert

    hAR ligand binding domain (AR Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 663-919

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28 E2 LBD-Tev-E1a-His

    Plasmid

    #232117

    Purpose

    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    DBT (DBT Human)
    

    Tags

    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pet28a-His6-Halo-Tev-Keap1

    Plasmid

    #62456

    Purpose

    His6-halo tagged human Keap1 protein for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Halo-Tev

    Expression

    Bacterial

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a(+) NRP1 b1b2 (273-586)

    Plasmid

    #162733

    Purpose

    Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

    Depositor

    Brett Collins

    Article

    Daly et al Science. 2020 Oct 20. pii: science.abd30

    Insert

    Human NRP1 b1b2 domains (residues 273-586)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Codon optimised

    Available Since

    March 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET28a-GGG-ELP(120nm)-Cys

    Plasmid

    #91572

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(120nm)-Cys
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET21-pelB-LaG-41-4GS-LaG-2

    Plasmid

    #172766

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41-4GS-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET co-transformation cloning vector (13S-A)

    Plasmid

    #48323

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET28a His tag-FRB-Venus C

    Plasmid

    #100980

    Purpose

    Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    His-FRB-Venus C
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28C-mCherry-SARS-CoV-2-Nucleocapsid

    Plasmid

    #204403

    Purpose

    E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. His-SUMO- flag-hDDI2-D252N_pET11a

    Plasmid

    #169123

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Mutation

    D252N

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET28a-Fgß-ddFLN4-HIS-ybbr

    Plasmid

    #101239

    Purpose

    wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    Fgß peptide, ddFLN4 fingerprint (FGB Human, D. discoideum)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET-45b-HOXD13(+7A)-IDR-mCherry

    Plasmid

    #145281

    Purpose

    Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET-Duet1_6xHis-TEV-GABARAP-Gly

    Plasmid

    #190930

    Purpose

    Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    GABARAP (GABARAP Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Ends with Glycine 116

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEW3: pET-32a Dot1L(2-416)

    Plasmid

    #124098

    Purpose

    Dot1L(2-416) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-416) (DOT1L Human)
    

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-mScarlet-TEV-Strep-His

    Plasmid

    #210823

    Purpose

    Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    mScarlet
    

    Tags

    TwinStrep, 10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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