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pEGFP-N1 Full length Importin beta

Plasmid

#106941

Purpose

Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

Depositor

Patrizia Lavia

Article

Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

Insert

human Importin beta-1 (KPNB1) (KPNB1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEB-WT-MYC (#647)

Plasmid

#99955

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-dTomato

Plasmid

#99376

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-dTomato

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(Hygro) myc-hPolQ-Flag

Plasmid

#73132

Purpose

To express in mammalian cells human PolQ

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

Polymerase theta (POLQ Human)

Tags

Flag and myc

Expression

Mammalian

Promoter

cmv promoter

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-BRD2

Plasmid

#65376

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD2 (BRD2 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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PP7_mCherry

Plasmid

#61763

Purpose

Expresses PP7 coat protein fused to mCherry

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

PP7 coat protein fused to mCherry

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

Ubc promoter

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Autobow

Plasmid

#45182

Depositor

Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pCAG-Autobow

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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tetO-ALN

Plasmid

#43918

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

6xHis and V5

Expression

Mammalian

Mutation

Gylcine-serine-glycine (GSG) linker followed by v…

Promoter

TRE promoter, Tet-ON

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP

Plasmid

#19444

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pShuttle-Cre-HA

Plasmid

#16583

Depositor

Bert Vogelstein

Article

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

cre recombinase (cre P1 bacteriophage)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-hPIASy

Plasmid

#15208

Depositor

Ke Shuai

Article

Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

Insert

PIASy (PIAS4 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA 14-3-3 sigma

Plasmid

#11946

Depositor

Michael Yaffe

Article

Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

Insert

Stratifin (SFN Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLBH531_MBP-Cas14a1 expression

Plasmid

#112500

Purpose

Expresses MBP fused to Cas14a1

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_WT_mCherry

Plasmid

#84020

Purpose

expression of SRSF2 in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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