4,133 results

1. CMV-GFP-NMHC II-A

   Plasmid

   #11347

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVHA hEZH2

   Plasmid

   #24230

   Purpose

   Mammalian expression of human EZH2 with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_smFP Myc

   Plasmid

   #59757

   Purpose

   encodes multiple copies of an epitope tag Myc

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _Myc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-Golgin84_VSVG-SBP-EGFP

   Plasmid

   #65305

   Purpose

   synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP VAMP2

   Plasmid

   #42308

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

   Insert

   VAMP 2
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.3_eGFP

   Plasmid

   #26822

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-eGFP-3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBI-MCS-EGFP

   Plasmid

   #16542

   Depositor

   Bert Vogelstein

   Article

   Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 HA-YY1

    Plasmid

    #104395

    Purpose

    HA-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1 (YY1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TetO-FUW-cMYC

    Plasmid

    #20324

    Depositor

    Rudolf Jaenisch

    Article

    Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

    Insert

    cMYC (Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. piRFP

    Plasmid

    #31857

    Purpose

    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

    Has Service

    Cloning Grade DNA

    Insert

    iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-P4M-SidM

    Plasmid

    #51469

    Purpose

    Biosensor for PtdIns4P

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Insert

    P4M-SidM(546-647)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-mDlx-GFP-Fishell-1

    Plasmid

    #83900

    Purpose

    GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    mDlx

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWZL hygro Flag HA TRAP220 wt

    Plasmid

    #17433

    Purpose

    Retroviral expression of Med1/TRAP220 in mammalian cells

    Depositor

    Kai Ge

    Article

    Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

    Insert

    TRAP220 (MED1 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-ERBB2

    Plasmid

    #23888

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

    Plasmid

    #141385

    Purpose

    Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-E (E SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pFA6a-TurboID-3MYC-natMX6 (pFB1434)

    Plasmid

    #126050

    Purpose

    For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

    Depositor

    François Bachand

    Article

    Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

    Insert

    TurboID
    

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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