We narrowed to 5,803 results for: SUP

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pCDNA4TO-3XFLAG Halo WT nsp1

Plasmid

#175412

Purpose

Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 nsp1 (ORF1ab CoV2)

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pF147 pSOD1A4VAcGFP1

Plasmid

#26408

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A4V

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
608 pSG5L HA RB del ex4

Plasmid

#10730

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del exon4 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of exon4

Available Since

Oct. 7, 2005

Availability

Academic Institutions and Nonprofits only
498 pSG5L HA RB del663 (NAAIRS)

Plasmid

#10728

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del663 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

residues 664 to 669 were replaced by NAAIRS, a fl…

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pNeuLite

Plasmid

#16247

Depositor

Mien-Chie Hung

Article

Xing et al Nat Med. 2000 Feb . 6(2):189-95.

Insert

Her-2/neu promoter (ERBB2 Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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Frt-hspB7

Plasmid

#63098

Purpose

Expression of HSPB7 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb7 (HSPB7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAcBac1-GFP(39TAG)

Plasmid

#217360

Purpose

Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

Enhanced green fluorescent protein

Use

Baculoviral

Expression

Insect and Mammalian

Mutation

Y39TAG

Promoter

CMV

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.1

Plasmid

#91895

Purpose

sgNf1.1 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.1

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk12_Untagged

Plasmid

#127176

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

None

Promoter

None

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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Cterminal HA Mios pRK5

Plasmid

#46329

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
GFP-alpha-E-Catenin 3xFP4 (895)

Plasmid

#67936

Purpose

Mammalian expression of alpha E catenin

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

alpha E catenin (Ctnna1 Mouse)

Tags

3xFP4 and EGFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
MSCV PIG C/EBPa

Plasmid

#66985

Purpose

Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Use

Retroviral

Promoter

LTR

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-hDIXDC1 WT

Plasmid

#61222

Purpose

Mammalian expression vector encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_S139D-GS3

Plasmid

#204748

Purpose

Expression of Cas9 and human H2AX with the S139D mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX S139D (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the S139D mutation

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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Flag WDR24 pLJM1

Plasmid

#46337

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Use

Lentiviral

Tags

FLag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pCsy_complex

Plasmid

#89232

Purpose

Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Inserts

Csy1
Csy2
Csy3
Csy4

Tags

6xHis-TEV protease

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDule2-3-nitroTyrosine (5B)

Plasmid

#85499

Purpose

Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

Insert

3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Expression

Bacterial

Mutation

Y32H H70C D158S I159A L162R

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-BaeI-Pgk-Cre

Plasmid

#173663

Purpose

Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

BaeI site

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-HKIImut

Plasmid

#99240

Purpose

Type II Hexokinase expressed in AS-30D rat hepatoma cell line

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 1997 Sep 5;272(36):22776-80

Insert

Hexokinase II (Hk2 Rat)

Expression

Mammalian

Mutation

Four silent mutations; P (115)L, A (192) V, F (78…

Promoter

CMV I.E.

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pF154 pcDNA3.1(+)SOD1G85R

Plasmid

#26400

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G85R

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-Puro-sgSNRK

Plasmid

#138694

Purpose

Expresses a human SNRK-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSNRK human (SNRK Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-FLAG-hDIXDC1-L-WT

Plasmid

#61210

Purpose

Entry clone encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Entr clone

Tags

FLAG

Promoter

None

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
T7-VEE-CFP

Plasmid

#193357

Purpose

Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7a-7

Plasmid

#56443

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab7a (RAB7A Human)

Tags

EGFP

Expression

Mammalian

Mutation

N117S in NM_004637.5

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Frt-V5-hspB7

Plasmid

#63108

Purpose

Expression of HSPB7 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb7 (HSPB7 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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VP-ARF

Plasmid

#89122

Purpose

mammalian expression of p14-hARF linked to VP16 activation domain

Depositor

Elizabeth Wilson

Article

Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

Insert

p14-hARF (CDKN2A Human)

Tags

VP16

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP11 CS puroR

Plasmid

#46748

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-GPD1

Plasmid

#32487

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1 (Gpd1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
LENTICRISPR-TKT_sgRNA2

Plasmid

#201625

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TKT (TKT Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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