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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 13,007 results for: cdna

Showing: 4921 - 4960 of 13007 results

  3. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  6. pcDNA3-PKCsub(T/A)-YPet-NES

    Plasmid
    #138208
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub(T/A)-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138208-plasmid-map-sequence-id-307411

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  10. pcDNA3.1-hRIPK1-Flag-K599R

    Plasmid
    #112490
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    K599R
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112490-plasmid-map-sequence-id-214537

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  12. pcDNA3.1-mouse cdk6 R31C

    Plasmid
    #75171
    Purpose
    Mammalian expression of mouse cdk6 with R31C
    Depositor
    Martine Roussel
    Insert
    cyclin dependent kinase 6 (Cdk6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R31C
    Promoter
    CMV
    Available since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  21. 835 pcDNA3 HA PTEN G129R

    Plasmid
    #10929
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G129R
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  31. pcDNA3.1-mCherry-CyrCR1.miR17

    Plasmid
    #128748
    Purpose
    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR.
    Depositor
    David Bartel
    Article
    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do
    Insert
    Cyrano conserved region (CR1) with miR-17 site replacing miR-7 site (OIP5-AS1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128748-plasmid-map-sequence-id-254120

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  34. pcDNAmyc-hPR-B-486-933

    Plasmid
    #89118
    Purpose
    mammalian expression of human progesterone receptor amino acid residues 486-933 with N-terminal myc tag, has PR DNA and ligand binding domains
    Depositor
    Elizabeth Wilson
    Article
    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6
    Insert
    human progesterone receptor ligand binding domain (PGR Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    contains aa 486-933
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89118-plasmid-map-sequence-id-175725

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  35. pcDNA5 rtTA BirA eIF4G

    Plasmid
    #158789
    Purpose
    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1
    Use
    Tags
    3xFlag and BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    miniCMV
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158789-plasmid-map-sequence-id-309804

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Showing: 4921 - 4960 of 13007 results