We narrowed to 32,062 results for: eng

1. pYPQ134-RZ-Cas12j2

   Plasmid

   #173930

   Purpose

   Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ265K

   Plasmid

   #173997

   Purpose

   Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   UNG-rAPOBEC1(R33A)-zCas9(D10A)
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   R33A in rAPOBEC1 and D10A in zCas9

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIALD2E

   Plasmid

   #185866

   Purpose

   Knocking out ALD6

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD(-125, 40)- ALD6(1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCS-Clover4-LUMABS-DEN1

   Plasmid

   #183487

   Purpose

   To express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.

   Depositor

   Jun Chu, Zongwen Jin

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-DEN1
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAID5WY

   Plasmid

   #165050

   Purpose

   Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Expression

   Yeast

   Mutation

   OsTIR1 is codon-optimised for expression in S. ce…

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ290-D977A

   Plasmid

   #129674

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Expression

   Plant

   Mutation

   D977A; AacCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ290-E848A

   Plasmid

   #129675

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Tags

   E848A; AacCas12b is rice codon optimized

   Expression

   Plant

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ291-D573A

   Plasmid

   #129676

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Expression

   Plant

   Mutation

   D573A; BthCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ291-D951A

   Plasmid

   #129677

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Expression

   Plant

   Mutation

   D951A; BthCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ291-E827A

    Plasmid

    #129678

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Expression

    Plant

    Mutation

    E827A; BthCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ292-D977A

    Plasmid

    #129680

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    D977A; AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ292-E848A

    Plasmid

    #129681

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    E848A; AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBTK224

    Plasmid

    #110590

    Purpose

    BTK Type 3 coding sequence for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pYPQ141-ZmUbi-RZ-Aa1.2

    Plasmid

    #136373

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ141-ZmUbi-RZ-Aa3.8.3

    Plasmid

    #136374

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ141-ZmUbi-RZ-Aac.3

    Plasmid

    #136377

    Purpose

    Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ141-ZmUbi-RZ-Aa1.2.3

    Plasmid

    #136372

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET51b(+)-LUCIDRS-MTX

    Plasmid

    #113925

    Purpose

    Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    LUCID_RS-MTX
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP

    Plasmid

    #99730

    Purpose

    overexpression of Tmeff2 and mCherry

    Depositor

    Mengsheng Qiu

    Article

    Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

    Insert

    Tmeff2 (Tmeff2 Mouse)
    

    Tags

    2xMyc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-CMV-Tmeff2-3xFlag-EF1-nlsCopGFP

    Plasmid

    #99729

    Purpose

    overexpression of Tmeff2 and CopGFP

    Depositor

    Mengsheng Qiu

    Article

    Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

    Insert

    Tmeff2 (Tmeff2 Mouse)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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