We narrowed to 7,151 results for: CIT

1. pRK5-LYCHOS (GPR155) ∆DEP-HA

   Plasmid

   #199683

   Purpose

   Transient expression of LYCHOS (GPR155) ∆DEP-HA

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   DEP domain deleted

   Promoter

   CMV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5 LYCHOS (GPR155) Y551A-HA

   Plasmid

   #199676

   Purpose

   Transient expression of LYCHOS (GPR155) Y551A mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y551 mutated to A

   Promoter

   CMV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a LZ LED WT-FLAG

   Plasmid

   #199679

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED WT (GPR155 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-LYCHOS (GPR155) P44A-HA

   Plasmid

   #199675

   Purpose

   Transient expression of LYCHOS (GPR155) P44A mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   P44 mutated to A

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-LYCHOS (GPR155) E48Q-HA

   Plasmid

   #199662

   Purpose

   Transient expression of LYCHOS (GPR155) E48Q mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E48 mutated to Q

   Promoter

   CMV

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-LYCHOS (GPR155) F43I-HA

   Plasmid

   #199674

   Purpose

   Transient expression of LYCHOS (GPR155) F43I mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F43 mutated to I

   Promoter

   CMV

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSPL3-hRPH3A_c.444Tmut

   Plasmid

   #190477

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.444G>T

   Promoter

   SV40

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSPL3-hRPH3A_c.1853Awt

   Plasmid

   #190478

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

   Plasmid

   #170236

   Purpose

   Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CHUK (CHUK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   S176E, S180E

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGMC00005

    Plasmid

    #166722

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGMC00006

    Plasmid

    #166723

    Purpose

    sgRNA against human ASNS

    Depositor

    Raj Chari

    Article

    Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

    Insert

    ASNS (ASNS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGG442

    Plasmid

    #165616

    Purpose

    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    

    Use

    CRISPR

    Tags

    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

    Expression

    Yeast

    Mutation

    Corrected homology relative to wild type SpCas9 (…

    Promoter

    TEF1

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus-Puma-4E-pEGFP-C1

    Plasmid

    #166746

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Puma-4E (BBC3 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Venus-tBid-2E-pEGFP-C1

    Plasmid

    #166747

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domain

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    tBid-2E (Bid Mouse)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    2 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 61427-sgHtt_10

    Plasmid

    #157861

    Purpose

    Expresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM system

    Depositor

    Xinyu Zhao

    Article

    Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

    Insert

    Htt (Htt Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 61427-sgHtt_6

    Plasmid

    #157860

    Purpose

    Expresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM system

    Depositor

    Xinyu Zhao

    Article

    Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

    Insert

    Htt (Htt Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

    Plasmid

    #105681

    Purpose

    Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    tac_Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

    Plasmid

    #105680

    Purpose

    Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    tac_Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MoFGFR31c-ires neo

    Plasmid

    #86496

    Purpose

    mamalian cell expression of FGFR3-FGFR1 chimera

    Depositor

    David Ornitz

    Article

    Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

    Insert

    FgfrR3-1 IIIc 15C7 (Fgfr1, Fgfr3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FGFR3-FGFR1 chimera

    Promoter

    Moloney murine leukemia virus LTR

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MoFGFR31b-ires neo

    Plasmid

    #86495

    Purpose

    mamalian cell expression of FGFR3-FGFR1 chimera

    Depositor

    David Ornitz

    Article

    Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

    Insert

    FgfrR31 IIIb 15C8 (Fgfr3, Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FGFR3-FGFR1 chimera

    Promoter

    Moloney murine leukemia virus LTR

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR221_SLC25A13

    Plasmid

    #132038

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A13 (SLC25A13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Rasgrf2-2A-dCre targeting vector

    Plasmid

    #61573

    Purpose

    Target a DHFR-destabilized Cre recombinase gene to the stop codon of the mouse Rasgrf2 gene

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Rasgrf2-2A-dCre (Rasgrf2 P1 bacteriophage, Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-FLEX-P301L Tau

    Plasmid

    #176267

    Purpose

    This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

    Depositor

    Tsuneya Ikezu

    Article

    You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

    Insert

    Flex-P301L tau (MAPT )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV phSyn1(S)-FlpO-bGHpA

    Plasmid

    #51669

    Purpose

    Can be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV Retrograde

    Insert

    FlpO recombinase gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid

    #65454

    Purpose

    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    H134R variant

    Promoter

    hSyn1

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCIG-FGF13A

    Plasmid

    #220793

    Purpose

    To express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    FGF13 (FGF13 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CROPseq-MPSV-eBFP2

    Plasmid

    #220472

    Purpose

    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

    Depositor

    Christian Krebs

    Article

    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSIREN-RetroQ-BPTF-sh5

    Plasmid

    #83045

    Purpose

    MMLV LTR plasmid pSIREN-RetroQ-BPTF-sh5

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #5 to BPTF (BPTF, Bptf Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge