We narrowed to 9,393 results for: Coli

1. pCL25

   Plasmid

   #15456

   Depositor

   Chia Lee

   Article

   Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

   Type

   Empty backbone

   Use

   S. aureus-e. coli shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426TEF-LptTPS-A

   Plasmid

   #89468

   Purpose

   Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production

   Depositor

   Jing-Ke Weng

   Insert

   LptTPS-A
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   Promoter

   pBR322 origin of replication for propagation in E…

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTCF200 Mouse beta-2 microglobulin

   Plasmid

   #180462

   Purpose

   E. coli expression of class I MHC light chain

   Depositor

   John Altman

   Insert

   Mouse beta-2 microglobulin
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTCF120 H-2D(b)

   Plasmid

   #180454

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   H-2D(b)
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PsbA2-HMGS-HMGR-ATOB-NPTI

   Plasmid

   #73338

   Purpose

   Construct expressing the upper MVA pathway in Synechocystis PCC 6803

   Depositor

   Anastasios Melis

   Insert

   HMGS-HMGR-ATOB-NPTI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   psbA2

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTCF3 H-2D(d)

   Plasmid

   #180452

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   H-2D(d)
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTCF170 H-2L(d)

   Plasmid

   #180458

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   H-2L(d)
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Sc FLAG-kin-mec3-pRSFDuet

   Plasmid

   #240741

   Purpose

   Over-express Sc FLAG-PKA kinase motif-mec3 in E. coli

   Depositor

   Michael O'Donnell

   Insert

   FLAG-kin-mec3 (MEC3 Budding Yeast)
   

   Use

   Tags

   3X FLAG followed by PKA kinase motif

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sc RFA[1+2]-pET

   Plasmid

   #240742

   Purpose

   Over-express Sc RFA1 & RFA2 in E. coli

   Depositor

   Michael O'Donnell

   Inserts

   RFA1 (RFA1 Budding Yeast)
   RFA2 (RFA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Sc RFC[2+3+4]-pET

    Plasmid

    #239203

    Purpose

    Overexpress Sc RFC2, RFC3, & RFC4 in E. coli

    Depositor

    Michael O'Donnell

    Inserts

    RFC2 (RFC2 Budding Yeast)
    RFC3 (RFC3 Budding Yeast)
    RFC4 (RFC4 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAIP4_6WEY

    Plasmid

    #234234

    Purpose

    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAIP4_3D1B

    Plasmid

    #234230

    Purpose

    Heterologous protein expression of RNA-induced transcriptional silencing complex protein tas3 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3D1B (tas3 Fission Yeast)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAIP4_2ESK

    Plasmid

    #234232

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2ESK (UBE2D2 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAIP4_5F66

    Plasmid

    #234226

    Purpose

    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5F66 (PPIA Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pAIP4_5HIH

    Plasmid

    #234205

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAIP4_5EIP

    Plasmid

    #234188

    Purpose

    Heterologous protein expression of RNA binding exosome specificity factor Mmi1 in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5EIP (mmi1 Fission Yeast)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAIP4_5KUW

    Plasmid

    #234183

    Purpose

    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5KUW (PPIA Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAIP4_3ONH

    Plasmid

    #234179

    Purpose

    Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3ONH (UBA2 Budding Yeast)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAIP4_6BIO

    Plasmid

    #234152

    Purpose

    Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6BIO (TVAG_298080 Trichomonas vaginalis)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAIP4_2H2Z

    Plasmid

    #234147

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pRD503

    Plasmid

    #220788

    Purpose

    Overexpresses HTkC in E. coli cells

    Depositor

    Remus Dame

    Article

    Schwab et al bioRxiv 2023.06.01.543357

    Insert

    HTkC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRD551

    Plasmid

    #220789

    Purpose

    Overexpresses HMfC in E. coli cells

    Depositor

    Remus Dame

    Article

    Schwab et al bioRxiv 2023.06.01.543357

    Insert

    HMfC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFHMSPN-avi-TEV-LIC V

    Plasmid

    #220885

    Purpose

    Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus expresssion

    Tags

    Honeybee melittin signal followed by His, avi and…

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pExp-AFV

    Plasmid

    #112560

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-AFV and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid

    #73440

    Purpose

    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC-P3A2-vioD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSET-iGluSnFR4f

    Plasmid

    #234444

    Purpose

    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

    Plasmid

    #169187

    Purpose

    To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX037

    Plasmid

    #167156

    Purpose

    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    caffeic acid cycle genes
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pNOS, p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFP.E222

    Plasmid

    #138248

    Purpose

    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSET-iGluSnFR4s

    Plasmid

    #234443

    Purpose

    Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOPINB-HOIL-1 full-length (1–510)

    Plasmid

    #193858

    Purpose

    Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

    Depositor

    Bernhard Lechtenberg

    Article

    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

    Insert

    HOIL-1 (RBCK1 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    Codon optimised for expression in E. coli

    Promoter

    T7

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCJ207

    Plasmid

    #162680

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid

    #107582

    Purpose

    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    GFP+
    

    Use

    Synthetic Biology; E. coli and bacillus shuttle v…

    Tags

    B. megaterium DSM319 xylA leader sequence (MTSSKI…

    Expression

    Bacterial

    Mutation

    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

    Promoter

    fba promoter Bacillus megaterium DSM319

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28(+)-NudC

    Plasmid

    #175022

    Purpose

    Purification of NudC enzyme

    Depositor

    Jeppe Vinther

    Article

    Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

    Insert

    nudC
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBGE3.0

    Plasmid

    #165988

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAav-TP53-T2A-BirA*

    Plasmid

    #115652

    Purpose

    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

    Depositor

    Sven Eyckerman

    Article

    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

    Inserts

    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    

    Use

    AAV

    Tags

    BirA* and T2A

    Expression

    Mutation

    Homology region 1 and Homology region 2

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge