We narrowed to 31,545 results for: Eng

1. HemH-R212A_pET-14b

   Plasmid

   #233262

   Purpose

   Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

   Depositor

   Andrew Emili, Mohan Babu

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   HemH (hemH )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 21…

   Promoter

   T7 promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pML484_RPL36-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227923

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RPL36 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227925

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227926

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227908

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   RAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227909

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   GPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227910

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227911

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   COG8 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pML494_ATP1B3-C-term_XTEN80-sfGFP-3xHA_pTMPLT

   Plasmid

   #227912

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   ATP1B3 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pML499_SLC3A2-C-term_XTEN80-sfGFP-3xHA_pTMPLT

    Plasmid

    #227913

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SLC3A2 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227914

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pML454_G3BP1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227915

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    G3BP1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227916

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227918

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227920

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    HSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227921

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227922

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227891

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    VPS35 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227892

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    EEA1 homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227893

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CLTA homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227894

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SEC61B homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227896

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227897

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    NCLN homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227900

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227901

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227902

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227903

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227904

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227905

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLJM1-NRBP1-FL-N-FLAG

    Plasmid

    #222675

    Purpose

    overexpressing full length NRBP1 with N-terminal FLAG

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    NRBP1 (NRBP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLJM1-TSC22D2-FL-N-FLAG

    Plasmid

    #222680

    Purpose

    overexpressing full length TSC22D2 with N-terminal FLAG

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    TSC22D2 (TSC22D2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLJM1-TSC22D2-FL-C-FLAG

    Plasmid

    #222681

    Purpose

    overexpressing full length TSC22D2 with C-terminal FLAG

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    TSC22D2 (TSC22D2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI

    Plasmid

    #196617

    Purpose

    Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-BJ.1
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pT2iApowriter2(#336)

    Plasmid

    #184104

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pT22Apowriter2(#340)

    Plasmid

    #184105

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT22iuasvApoWtEos3N(#486)

    Plasmid

    #184107

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT22iuasvApoWChe(#488)

    Plasmid

    #184108

    Purpose

    cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVRC8400-SARS-CoV-2-RBD-SD1-C.37-AVI

    Plasmid

    #181702

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-C.37 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452Q, F490S

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVRC8400-SARS-CoV-2-RBD-SD1-B.1.621-AVI

    Plasmid

    #181703

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-B.1.621 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    R346K, E484K, N501Y

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pVRC8400-SARS-CoV-2-S2P-B.1.621-AVI

    Plasmid

    #181694

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.621 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T95I, insert144T, Y144S, Y145N, R346K, E484K, N50…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pVRC8400-SARS-CoV-2-NTD-C.37-AVI

    Plasmid

    #181696

    Purpose

    Expresses SARS-CoV-2 NTD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-C.37 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G75V, T76I, R246N, ∆S247-∆D253,

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pVRC8400-SARS-CoV-2-NTD-B.1.621-AVI

    Plasmid

    #181697

    Purpose

    Expresses SARS-CoV-2 NTD domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-B.1.621 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T95I, insert144T, Y144S, Y145N

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pVRC8400-SARS-CoV-2-RBD-C.37-AVI

    Plasmid

    #181699

    Purpose

    Expresses SARS-CoV-2 RBD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-C.37 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452Q, F490S

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pVRC8400-SARS-CoV-2-RBD-B.1.621-AVI

    Plasmid

    #181700

    Purpose

    Expresses SARS-CoV-2 RBD domain from the B.1.621 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-B.1.621 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    R346K, E484K, N501Y

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pVRC8400-SARS-CoV-2-S2P-C.37-AVI

    Plasmid

    #181693

    Purpose

    Expresses SARS-CoV-2 S2P protein from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-C.37 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    G75V, T76I, R246N, ∆S247-∆D253, L452Q, F490S, D61…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pVRC8400-SARS-CoV-2-RBD-SD1-N501Y-A570D-AVI

    Plasmid

    #176449

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing N501Y-A570D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-N501Y-A570D (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    N501Y, A570D

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-AVI

    Plasmid

    #176452

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-L452R (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452R

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pVRC8400-SARS-CoV-2-RBD-SD1-S477N-AVI

    Plasmid

    #176453

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-S477N (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    S477N

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pVRC8400-SARS-CoV-2-RBD-SD1-E484K-AVI

    Plasmid

    #176454

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-E484K (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    E484K

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-E484Q-AVI

    Plasmid

    #176455

    Purpose

    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-E484Q mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-RBD-SD1-L452R-E484Q (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L452R, E484Q

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge