We narrowed to 8,601 results for: Ott

1. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

   Plasmid

   #208291

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpRY(D10A/…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-ABE8e-SpRY-HiFi-P2A-EGFP (LM458)

   Plasmid

   #197508

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpRY-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpRY-HiFi(D10A/A61R/…

   Promoter

   CMV and T7

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPHAGE_DLD-H450A-C-TAP

   Plasmid

   #83478

   Purpose

   Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   H450A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPHAGE_DLD-S456A-C-TAP

   Plasmid

   #83479

   Purpose

   Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   S456A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

   Plasmid

   #223079

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX_307-SOD1WT

   Plasmid

   #83444

   Purpose

   Mammalian expression of SOD1WT

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

   Plasmid

   #223115

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

   Plasmid

   #223116

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Rosa26-LSL-PE2 Targeting Vector

   Plasmid

   #196038

   Purpose

   The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.

   Depositor

   Tyler Jacks

   Article

   Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

   Insert

   Cas9-MMLV-RT
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid

    #193859

    Purpose

    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli

    Depositor

    Bernhard Lechtenberg

    Article

    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

    Insert

    HOIL-1 (RBCK1 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    Residues 233–510, codon optimised for expression …

    Promoter

    T7

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT/TO-FLAG-SHLD2

    Plasmid

    #114117

    Purpose

    Expresses N-terminally tagged FLAG-SHLD2 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Genomic integration, tetracycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCW-eGFP-FHA-SHLD2

    Plasmid

    #114121

    Purpose

    Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    RNF8 FHA domain (aa 2-160) and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pPHAGE-CR-PINK1-C-TAP

    Plasmid

    #128510

    Purpose

    Lentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PTEN induced kinase 1 (PINK1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA tags

    Expression

    Mammalian

    Mutation

    Synonymous silent mutation in Q5 to render sgRNA …

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMV-DIO-Magneto2.0-sNRPpA

    Plasmid

    #74307

    Purpose

    Cre-dependent expression of Magneto2.0 in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)
    

    Use

    AAV and Cre/Lox

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    TRPV4: delta 760-871

    Promoter

    CMV (after Cre recombination)

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS426-HsFECH

    Plasmid

    #174524

    Purpose

    Expresses human ferrochelatase (FECH) in yeast

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    FECH with yeast HEM15 promoter and terminator (FECH Budding Yeast, Human, Synthetic)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Lacks original mitochondrial targeting sequence (…

    Promoter

    HEM15 (yeast, 257 bp)

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

    Plasmid

    #52505

    Purpose

    mammalian expression vector of 53BP1 28A mutant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    53BP1 28A (TP53BP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    All 28 SQ/TQ motifs in N-term of 53BP1 have been …

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pLEX_307-SOD1E133insTT

    Plasmid

    #83446

    Purpose

    Mammalian expression of SOD1E133insTT.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E133insTT (after GA)

    Promoter

    EF-1α

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCW-eGFP-SHLD2-m1

    Plasmid

    #114120

    Purpose

    Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (m1 mutant) (SHLD2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    W489A, F494A, W495A mutations introduced

    Promoter

    TRE promoter, Tet ON

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCW-eGFP-FHA

    Plasmid

    #114129

    Purpose

    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    aa 2-160

    Promoter

    TRE promoter, Tet ON

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA

    Plasmid

    #74302

    Purpose

    Expression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafish

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTL, FTH1, Trpv4 Rat, Human)
    

    Use

    Zebrafish

    Tags

    FLAG, cmcl2::EGFP, and p2A-mCherry

    Expression

    Mutation

    TRPV4: delta 760-871

    Promoter

    Neurog1

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

    Plasmid

    #74306

    Purpose

    Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Inserts

    TRPV4 (Trpv4 Rat)
    ferritin (FTL and HTL1) (FTL, FTH1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG and p2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (after cre recombination)

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

    Plasmid

    #194015

    Purpose

    Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(Oxtr.1)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and u6

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGLUE-SHLD2

    Plasmid

    #114118

    Purpose

    Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Tags

    HA tag, Streptavidin-binding tag (Streptag), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pExp-His-zBasic-RBD

    Plasmid

    #194999

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli

    Depositor

    Marko Hyvönen

    Article

    Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Tags

    His8-Zbasic

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLEX_307-SOD1E133Δ

    Plasmid

    #83445

    Purpose

    Mammalian expression of SOD1E133Δ.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E133Δ

    Promoter

    EF-1α

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

    Plasmid

    #162878

    Purpose

    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLENTI-PGK-HYGRO-GATA6mut-HA

    Plasmid

    #65476

    Purpose

    Lentiviral expression of GATA6 Znc-finger mutant

    Depositor

    Eduard Batlle

    Article

    Whissell et al Nat Cell Biol. 2014 Jul;16(7):695-707. d

    Insert

    GATA6mut (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    lacking the eight most highly conserved bases of …

    Promoter

    PGK

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pSOD1E133K-AcGFP1

    Plasmid

    #83442

    Purpose

    Expresses SOD1E133K in mammalian cells.

    Depositor

    Mohan Babu

    Article

    Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    E133K

    Promoter

    CMV

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV[Exp]-Neo-CMV>hCCNL2

    Plasmid

    #239219

    Purpose

    Expresses CCNL2 in mammalian cell lines

    Depositor

    Jason Sheltzer

    Article

    Julian et al bioRxiv 2025.08.03.668359; doi: https://

    Insert

    cyclin L2 (CCNL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV[Exp]-Bsd-CMV>hCDK11B

    Plasmid

    #239211

    Purpose

    Expresses CDK11B in mammalian cell lines

    Depositor

    Jason Sheltzer

    Article

    Julian et al bioRxiv 2025.08.03.668359; doi: https://

    Insert

    cyclin dependent kinase 11B (CDK11B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV[Exp]-Bsd-CMV>hCDK11A G567S

    Plasmid

    #239214

    Purpose

    Expresses CDK11A with G567S resistance mutation in mammalian cell lines

    Depositor

    Jason Sheltzer

    Article

    Julian et al bioRxiv 2025.08.03.668359; doi: https://

    Insert

    cyclin dependent kinase 11A with G567S resistance mutation (CDK11A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glycine 567 to Serine

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-T7-ABE8e-SpCas9(ARGIMR)-P2A-EGFP (AHK507)

    Plasmid

    #223118

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(ARGIMR)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/ARGIMR)=D1135A, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-T7-ABE8e-SpCas9(LWQYQH)-P2A-EGFP (AHK504)

    Plasmid

    #223119

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWQYQH amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWQYQH)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWQYQH)=D1135L, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-T7-ABE8e-SpCas9(MWKYQS)-P2A-EGFP (AHK505)

    Plasmid

    #223120

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MWKYQS)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-T7-ABE8e-SpCas9(MWKYQA)-P2A-EGFP (AHK503)

    Plasmid

    #223122

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MWKYQA amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MWKYQA)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MWKYQA)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-T7-ABE8e-SpCas9(KWRQLC)-P2A-EGFP (RAS3467)

    Plasmid

    #223112

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(KWRQLC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/KWRQLC)=D1135K, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-T7-ABE8e-SpCas9(MRKCRS)-P2A-EGFP (BKS1034)

    Plasmid

    #223117

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MRKCRS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MRKCRS)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

    Plasmid

    #223102

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)

    Plasmid

    #223103

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-T7-SpCas9(MRKQKC)-P2A-EGFP (RAS2838)

    Plasmid

    #223104

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKQKC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKQKC)=D1135M, S1136R, G1218K, E1219Q, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCMV-T7-SpCas9(MRKSKC)-P2A-EGFP (RAS2840)

    Plasmid

    #223105

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKSKC)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKSKC)=D1135M, S1136R, G1218K, E1219S, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid

    #223106

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid

    #223107

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid

    #223108

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid

    #223109

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCMV-T7-SpCas9(KWKYES)-P2A-EGFP (RAS2900)

    Plasmid

    #223095

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWKYES amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KWKYES)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KWKYES)=D1135K, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCMV-T7-SpCas9(LWKYEY)-P2A-EGFP (RAS3001)

    Plasmid

    #223096

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYEY amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKYEY)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKYEY)=D1135L, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCMV-T7-SpCas9(MKACKV)-P2A-EGFP (AHK136)

    Plasmid

    #223097

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKACKV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MKACKV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MKACKV)=D1135M, S1136K, G1218A, E1219C, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCMV-T7-SpCas9(MKRCKS)-P2A-EGFP (AHK140)

    Plasmid

    #223098

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MKRCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MKRCKS)=D1135M, S1136K, G1218R, E1219C, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCMV-T7-SpCas9(KRRCKV)-P2A-EGFP (RAS3014)

    Plasmid

    #223099

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KRRCKV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRRCKV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(KRRCKV)=D1135K, S1136R, G1218R, E1219C, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge