We narrowed to 3,857 results for: SPL;

1. Rtn2b-RHD272-472-mNeon

   Plasmid

   #186605

   Purpose

   Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Met-Gly-Ser-Lys peptide added at N-terminus for c…

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. IBMc227

   Plasmid

   #161948

   Purpose

   Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. IBMc367

   Plasmid

   #161947

   Purpose

   Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. IBMc228

   Plasmid

   #161946

   Purpose

   Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHBS1385 TDP43 RRM-GFP VLIM-S

   Plasmid

   #118792

   Purpose

   To test the effect of sequence on TDP43 RRM-GFP droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All VLIM to S in CTD of TDP43 RRM-GFP reporter

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHBS1384 TDP43 RRM-GFP FYW-L

   Plasmid

   #118793

   Purpose

   To test the effect of sequence on TDP43 RRM-GFP droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All FYW to L in CTD of TDP43 RRM-GFP reporter

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_SNORD2 probe

   Plasmid

   #73070

   Purpose

   antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD2 (SNORD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1_SNORD60 probe

   Plasmid

   #73071

   Purpose

   antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD60 (SNORD60 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pHBS1292 TDP43 RRM-GFP G368W+W385G

   Plasmid

   #107838

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G368W and W385G mutations in LLPS reporter

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHBS1039 TDP43 RRM-GFP 2xΦ

    Plasmid

    #107806

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to two clusters in LLPS…

    Promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHBS1038 TDP43 RRM-GFP 4xΦ

    Plasmid

    #107805

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to four clusters in LLP…

    Promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHBS1037 TDP43 RRM-GFP 6xΦ

    Plasmid

    #107804

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to six clusters in LLPS…

    Promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMYA5T2/pDEST17

    Plasmid

    #98118

    Purpose

    Express CMYA5 (aa 4039 – 4069) Leu allele (4063) witha N-terminal 6x-His tag in bacteria

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    rs10043986 (NP_705838.3:p.Pro4063Leu)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. CMYA5T/pACT2

    Plasmid

    #97209

    Purpose

    CMYA5 (aa 4039 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybrid

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    rs10043986 (NP_705838.3:p.Pro4063Leu)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. p198 Dync1i2.F (Ex1a)

    Plasmid

    #26448

    Depositor

    Elizabeth Fisher

    Article

    Kuta et al PLoS One. 2010 . 5(7):e11682.

    Insert

    cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)
    

    Use

    Subcloning and sequencing

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    March 24, 2011

    Availability

    Academic Institutions and Nonprofits only

16. p414GPD-3xFLAG-Nop4 L306P

    Plasmid

    #169262

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 L306P (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    p.(Leu306Pro)

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. p414GPD-3xFLAG-Nop4 dE5

    Plasmid

    #169263

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E5 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 5

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. p414GPD-3xFLAG-Nop4 dE8

    Plasmid

    #169264

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E8 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 8

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. p414GPD-3xFLAG-RBM28 L351P

    Plasmid

    #169266

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 L351P (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    p.(Leu351Pro)

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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20. p414GPD-3xFLAG-RBM28 dE5

    Plasmid

    #169267

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 delta E5 (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted Exon 5

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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