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pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

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pLenti-mCherry-Mango II x 24

Plasmid

#127587

Purpose

Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

Depositor

David Rueda

Publication

Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

Insert

mCherry-Mango II x24

Tags

mCherry

Expression

Mammalian

Promoter

pTRE Tet ON

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Cbh_v5 AAV-ABE N-terminal

Plasmid

#137177

Purpose

AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-ABE N-terminal

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Publication

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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tetO.Sox9.Puro

Plasmid

#117269

Purpose

Expresses Sox9 and puromycin resistance gene under control of tetO sequence.

Depositor

Henrik Ahlenius

Publication

Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

T2A and puromycin resistant cassette (C terminal …

Expression

Mammalian

Promoter

tet-on

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pGEMHE-mCherry-mTrim21

Plasmid

#105522

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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lentiGuide-Hygro-dTomato

Plasmid

#99376

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-dTomato

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pRRL_SRSF2_WT_mCherry

Plasmid

#84020

Purpose

expression of SRSF2 in mammalian cells

Depositor

Robert Bradley

Publication

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCW57-MCS1-P2A-MCS2 (GFP)

Plasmid

#80924

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Publication

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Publication

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Dendra2-Lifeact-7

Plasmid

#54694

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lifeact-Dendra2

Expression

Mammalian

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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tetO-ALN

Plasmid

#43918

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

6xHis and V5

Expression

Mammalian

Mutation

Gylcine-serine-glycine (GSG) linker followed by v…

Promoter

TRE promoter, Tet-ON

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3 IKKe flag

Plasmid

#26201

Depositor

Tom Maniatis

Publication

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

I-kappa-B kinase epsilon (IKBKE Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Publication

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

UBF (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Flag-hPIASy

Plasmid

#15208

Depositor

Ke Shuai

Publication

Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

Insert

PIASy (PIAS4 Human)

Tags

Flag

Expression

Mammalian

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pEGFP-N1-ACTR3

Plasmid

#8462

Depositor

Matthew Welch

Publication

Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACTR3 (ACTR3 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3 HA 14-3-3 sigma

Plasmid

#11946

Depositor

Michael Yaffe

Publication

Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Stratifin (SFN Human)

Tags

HA

Expression

Mammalian

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3 HA Arf6 ActQ67L

Plasmid

#10835

Depositor

Thomas Roberts

Publication

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arf6 Q67L (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L. Defective in GTP hydrolysis. Constitutive…

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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