4,181 results

1. pBAM1

   Plasmid

   #60487

   Purpose

   This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. iCas

   Plasmid

   #84232

   Purpose

   Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

   Depositor

   Meng How Tan

   Article

   Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

   Insert

   Cas9
   

   Tags

   2A-OFP co-expression and ERT2-ERT2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-LC3

   Plasmid

   #11546

   Depositor

   Karla Kirkegaard

   Article

   Jackson et al PLoS Biol. 2005 May . 3(5):e156.

   Insert

   LC3B (MAP1LC3B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-Puro-MEK-DD

   Plasmid

   #15268

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   Map2k1 (Mapk1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S218D S222D

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-multi-Guide

   Plasmid

   #85401

   Purpose

   Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas13d-dsRBD-APEX2

   Plasmid

   #154939

   Purpose

   TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-FLAG-Ku80

   Plasmid

   #46958

   Purpose

   Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku80 (XRCC5 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-Kras G12C

    Plasmid

    #58901

    Purpose

    Expresses human Kras with a G12C mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12C mutation

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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