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R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

Plasmid

#159579

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-YTHDF2

Plasmid

#38089

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

YTHDF2 YTH domain family, member 2 (YTHDF2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-HNRNPR

Plasmid

#38067

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

HNRNPR heterogeneous nuclear ribonucleoprotein R (HNRNPR Human)

Tags

FLAG, HA, and His

Expression

Mammalian

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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KHBD00297

Plasmid

#34423

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10798 (Myc Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
hCXCR4-LSSmOrange

Plasmid

#110197

Purpose

Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange FP

Depositor

Sergi Padilla Parra

Article

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Insert

hCXCR4 (CXCR4 Human)

Tags

LSSmOrange

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX2-1-178-EGFP-Hygro

Plasmid

#238094

Purpose

Expression of structurally predicted WWE domain of DTX2 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX2 (DTX2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-178 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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WP_010880027.1

Plasmid

#194729

Purpose

Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

Depositor

Fang Li

Article

Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

Insert

nanoparticle-proteinA and His tag

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-KIAA1967

Plasmid

#38082

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

KIAA1967 (CCAR2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR(Y107A)-Hygro

Plasmid

#176073

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR (Y107A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation to convert Try107 to Ala

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pYTRW26K_1Ti1

Plasmid

#177292

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Plasmid

#159580

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2113

Plasmid

#49138

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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R2pH-LAMPx3ALFA

Plasmid

#225136

Purpose

R2pH-LAMP1-labelled lysosomes with 3xLFA

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Zebrafish aBb crystallin 2kb promoter/AcGFP

Plasmid

#98099

Purpose

Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

Plasmid

#122850

Purpose

Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

m(KS_MAT)_mouseL_ACP (Fasn Mouse)

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSK+ chickBMPR-1B CA

Plasmid

#49528

Purpose

cloning vector for constitutively active chick BMP receptor 1B

Depositor

Lee Niswander, Peter ten Dijke

Article

Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

Insert

BMPR-1B CA (BMPR1B Chicken)

Expression

Bacterial

Mutation

constitutively active mutation (Q203D); also P2A,…

Promoter

T3

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-ERF

Plasmid

#98626

Purpose

Gateway entry TOPO TA cloning vector ERF

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ERF (Erf Mouse)

Use

Topo ta cloning vector

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

Plasmid

#154775

Purpose

plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAA in GFP reporter, UUA anticodon and G10A, …

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-EDF1

Plasmid

#38079

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

EDF1 endothelial differentiation-related factor 1 (EDF1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-deltaLRR-cSSH

Plasmid

#220210

Purpose

Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Mutation

c.4-75del / p.2-24del

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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