We narrowed to 162,408 results for: addgene

1. pYES2-HTH-HHT2

   Plasmid

   #86482

   Purpose

   yeast expression of HHT2 with His10-TEV-HA tag

   Depositor

   Akash Gunjan

   Article

   Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

   Insert

   HHT2 (HHT2 Budding Yeast)
   

   Use

   Tags

   HIS10-TEV-HA (HTH)

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. EdTP

   Plasmid

   #24311

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   31 first amino acids of hTcf4 have been deleted.

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. tdTomato-LacI

   Plasmid

   #183921

   Purpose

   Lac repressor labeled with tdTomato; can be used to label lacO sites

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdTomato-LacI (lacI Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2-cryR;cldn5bP1Egfp

   Plasmid

   #90158

   Purpose

   contains cldn5b specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   cldn5bP1Egfp (cldn5b Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-eDHFR

   Plasmid

   #107266

   Purpose

   mCherry-eDHFR in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   mCherry-eDHFR (dhfr E.coli)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 Rheb1 shRNA #1

   Plasmid

   #26625

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Rheb1 shRNA (RHEB Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

7. MAP2-GCaMP3

   Plasmid

   #43917

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Insert

   GCaMP3
   

   Use

   Lentiviral

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   E247V in GCaMP3

   Promoter

   Human MAP2 promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVPT-rtTR-KRAB-2SM2

   Plasmid

   #11652

   Purpose

   Tet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, rtTR-KRAB-2SM2, Tet-off
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-Flex-rev-oChIEF-tdTomato

   Plasmid

   #30541

   Purpose

   Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

   Depositor

   Rory McQuiston

   Article

   Bell et al Neuropharmacology. 2011 Aug 25.

   Insert

   oChIEF-tdtomato
   

   Use

   AAV and Cre/Lox

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. E[beta]P

    Plasmid

    #24313

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGH153_MS2-AIDΔ-Hygro

    Plasmid

    #85416

    Purpose

    lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AIDΔ and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. HLA_B5101

    Plasmid

    #165011

    Purpose

    Expression of HLA-B*51:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-B*51:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

    Plasmid

    #136262

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. AviTag-SpyCatcher

    Plasmid

    #72326

    Purpose

    Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    AviTag-SpyCatcher
    

    Use

    Tags

    N-term His6 tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSyn-IRES2-GFP

    Plasmid

    #234875

    Purpose

    Lentiviral plasmid with a multiple cloning site followed by IRES2-GFP.

    Depositor

    Andrew Arrant

    Article

    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSYn

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRG705_lexO-AsiSI_LexA-TF_LEU2MX

    Plasmid

    #154817

    Purpose

    Inducible expression of AsiSI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AsiSI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 Rheb1 shRNA #2

    Plasmid

    #26626

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Rheb1 shRNA (RHEB Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. Tol2 8xSTAR-sTomato-NLS. PGK-puro

    Plasmid

    #136261

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. H2B-MiniR

    Plasmid

    #208328

    Purpose

    MiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    H2B-MiniR
    

    Use

    Tags

    MiniR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PNCA-GFP-dYY1

    Plasmid

    #67791

    Purpose

    Use together with pCMV-intron and VSV-G to package MMLV reporter virus

    Depositor

    Stephen Goff

    Article

    Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

    Insert

    MMLV-LTR-GFP
    

    Use

    Tags

    Expression

    Mutation

    Deletion of LTR U3 YY1 binding domain

    Promoter

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only