We narrowed to 10,816 results for: HA

1. pCMV-MBP-yATOM_WDR5

   Plasmid

   #209706

   Purpose

   Mammalian expression of y-ATOM biosensor for WDR5

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   MBP-yATOM_WDR5
   

   Use

   Tags

   MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-MBP-yATOM_SH2

   Plasmid

   #209708

   Purpose

   Mammalian expression of y-ATOM biosensor for SH2

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   MBP-yATOM_SH2
   

   Use

   Tags

   MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-PRLSS-yATOM_SH2-KDEL

   Plasmid

   #209714

   Purpose

   Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-yATOM_SH2-KDEL
   

   Use

   Tags

   PRLSS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-MBP-yATOM_WDR5 (R80A)

   Plasmid

   #209713

   Purpose

   Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   MBP-yATOM_WDR5 (R80A)
   

   Use

   Tags

   MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

   Plasmid

   #209718

   Purpose

   Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-NbGFP_miRFP670nano3-KDEL
   

   Use

   Tags

   PRLSS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-PRLSS-Clover-KDEL

   Plasmid

   #209720

   Purpose

   Mammalian expression of clover localized to endoplasmic reticulum

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   PRLSS-Clover-KDEL
   

   Use

   Tags

   PRLSS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-Cox4-yATOM_SH2

   Plasmid

   #209715

   Purpose

   Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   Cox4-yATOM_SH2
   

   Use

   Tags

   Cos4

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KBB382

   Plasmid

   #185085

   Purpose

   HA-tagged POM152 with glycosylation sites mutated

   Depositor

   Ken Belanger

   Article

   Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

   Insert

   POM152
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pT22i5uasH3En2SE4P3Che(#1212)

   Plasmid

   #184098

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Insert

   3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Expression

   Mutation

   chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…

   Promoter

   5xUAS

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pColE1-AlphaOmega

    Plasmid

    #165867

    Purpose

    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 6xHis(TEV)-yDrp1-StrepII

    Plasmid

    #174427

    Purpose

    Expresses S. pombe Drp1 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+-wbp2nlT45A

    Plasmid

    #172464

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlT45A
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Threonine at aa45 to Alanine

    Promoter

    SP6

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBlastR-GMRWhite

    Plasmid

    #165885

    Purpose

    Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165888

    Purpose

    Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

    Plasmid

    #165889

    Purpose

    Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    All-in-one FC31 RMCE Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGal4BD 1-147

    Plasmid

    #165819

    Purpose

    1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Gal4BD 1-147
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAL4AD 768-881

    Plasmid

    #165820

    Purpose

    768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    GAL4AD 768-881
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pM80

    Plasmid

    #173173

    Purpose

    HA-dCas9

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWSK29-wzzBST

    Plasmid

    #172968

    Purpose

    pWSK29 with Salmonella typhimurium wzzBST gene

    Depositor

    Renato Morona

    Article

    Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

    Insert

    wzzB gene from Salmonella enterica serovar Typhimurium
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K198E- please see depositor comments

    Promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWSK29-fepEST

    Plasmid

    #172969

    Purpose

    pWSK29 with Salmonella typhimurium fepEST gene

    Depositor

    Renato Morona

    Article

    Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

    Insert

    fepE gene from Salmonella enterica serovar Typhimurium
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G160S- please see depositor comments

    Promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. C514-E59: attB5r-RU5u-2xHA-nostop-attB1r

    Plasmid

    #162966

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Dual HA epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGMR-WhiteA2

    Plasmid

    #165874

    Purpose

    Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Eye Marker Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPuroR-GMRWhite

    Plasmid

    #165884

    Purpose

    Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

    Plasmid

    #162805

    Purpose

    STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI-HA_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWDY1226

    Plasmid

    #138593

    Purpose

    Expresses NatL2-R156A in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    changed Arginine 156 to Alanine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWDY1222

    Plasmid

    #138592

    Purpose

    Expresses NatL2-K418A in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    changed Lysine 418 to Alanine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMET17p-CCG 316

    Plasmid

    #131166

    Purpose

    For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

    Type

    Empty backbone

    Use

    Tags

    CCG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBS_KS_attB2_SA(2)_T2AGAL80

    Plasmid

    #125219

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL80
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

    Plasmid

    #91945

    Purpose

    Bacterial expression of maize SAE1 and truncated SAE2

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    HA-ZmSAE1
    ZmSAE2-FLAG
    

    Use

    Tags

    FLAG and HA

    Expression

    Bacterial

    Mutation

    Truncation of ZmSAE2 to mimic a splice variant

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNCMV HPV8 E6 R138S

    Plasmid

    #87659

    Purpose

    Flag/HA tagged HPV8 E6 R138S

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    R138S

    Promoter

    CMV

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNCMV HPV8 E6 K64A

    Plasmid

    #87656

    Purpose

    Flag/HA tagged HPV8 E6 K64A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    K64A

    Promoter

    CMV

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNCMV HPV8 E6 K136N

    Plasmid

    #87657

    Purpose

    Flag/HA tagged HPV8 E6 K136N

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    K136N

    Promoter

    CMV

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNCMV HPV8 E6 K142A

    Plasmid

    #87660

    Purpose

    Flag/HA tagged HPV8 E6 K142A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    K142A

    Promoter

    CMV

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNCMV HPV8 E6 L59D

    Plasmid

    #87655

    Purpose

    Flag/HA tagged HPV8 E6 L59D

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    L59D

    Promoter

    CMV

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NCMV MmuPV1 E6 R52A

    Plasmid

    #87678

    Purpose

    Flag/HA tagged MmuPV1 E6 R52A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    MmuPV1 E6
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mutation

    R52A

    Promoter

    CMV

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNCMV MmuPV1 E6 R130A

    Plasmid

    #87654

    Purpose

    Flag/HA tagged MmuPV1 E6 R130A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    MmuPV1 E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    R130A

    Promoter

    CMV

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNCMV MmuPV1 E6

    Plasmid

    #87653

    Purpose

    Flag/HA tagged MmuPV1 E6

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    MmuPV1 E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. P. falciparum DHPS

    Plasmid

    #64080

    Purpose

    Expression of P. falciparum dihydropteroate synthase

    Depositor

    Vern Schramm

    Article

    Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

    Insert

    dihydropteroate synthase
    

    Use

    Tags

    Histidine tag

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. S. aureus DHPS

    Plasmid

    #64079

    Purpose

    Expression of Staphylococcus aureus dihydropteroate synthase

    Depositor

    Vern Schramm

    Article

    Stratton et al ACS Chem Biol. 2015 Oct 16;10(10):2182-6

    Insert

    dihydropteroate synthase
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

40. TM1442-pET-21a

    Plasmid

    #12650

    Depositor

    SeWon Suh

    Article

    Lee et al Proteins. 2004 Jul 1. 56(1):176-9.

    Insert

    Putative anti-sigma factor antagonist (TM1442 T. maritima)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge