We narrowed to 5,866 results for: org
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Plasmid#118897Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEVDepositorInsertNcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with TEVprotease and TwinStrep-MB…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_ST-SBP-mCherry
Plasmid#65263Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST
Plasmid#164235Purposelentiviral plasmid for GFP-TREX1 delta N (aa236-314)DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationdeletion of amino acids 1-234PromoterCMVAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 H83L H86L
Plasmid#12211DepositorInsertPak1 (PAK1 Human)
TagsMycExpressionMammalianMutationPBD (p21-binding domain) mutant: H83L, H86L (see …Available SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry
Plasmid#67668PurposeZebrafish expression vector for ubiquitous expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2Available SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1C
Plasmid#196685PurposeRep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationPTQGTLR insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_ATP1A1_G7_Dual_sgRNA
Plasmid#173206PurposeCoselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.DepositorInsertATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
UseCRISPRExpressionMammalianPromoterDual U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECFP-C1-hSERT
Plasmid#70104Purposemammalian expression of hSERT with N-terminal CFP fusionDepositorAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialPromoterPrhaAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Flag-E2-crimson.IRES-puro.WPRE (CG568)
Plasmid#139445PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from SFFV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianPromoterSFFVAvailable SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mRuby-Rab8a
Plasmid#194337PurposeExpresses mRuby Rab8a in mammalian cells and zebrafish embryosDepositorAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PH-SWAP70
Plasmid#84582PurposeExpresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2DepositorInsertSWAP70 residues 209-306 (SWAP70 Human)
TagsmCherryExpressionMammalianMutationPleckstrin-homology domain, residues 209-306PromoterCMVAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pASPIre3
Plasmid#154844PurposeDerivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this studyDepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
ExpressionBacterialAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-mCherry_puromycin
Plasmid#65285PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianPromoterCMVAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellulose
Plasmid#36300DepositorInsertpCellulose
UseSynthetic BiologyPromoterTwo promoters from the E coli genome drive two di…Available SinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOCC119
Plasmid#118884Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease and mGFP, cl…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266
Plasmid#164713PurposeGateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editingDepositorInsertzCas9(D10A)-PmCDA1-UGI
UseCRISPR; Gateway compatible pmcda1-cbe_v01 entry c…ExpressionPlantAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/mCherry-pBirA-HA
Plasmid#154458Purposeexpresses mCherry-pBirA-HA/biochemical assayDepositorInsertmCherry-pBirA-HA
ExpressionMammalianPromoterCMV-F, T7 promoterAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only