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We narrowed to 22,079 results for: his

Showing: 6401 - 6450 of 22079 results

  3. pGMCtKq28-TSC10M1-sspB

    Plasmid
    #49526
    Purpose
    Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    TSC10M1-sspB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49526-plasmid-map-sequence-id-73918

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  4. pGMCtN-TSC10M1-sspB

    Plasmid
    #49521
    Purpose
    Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    TSC10M1-sspB
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49521-plasmid-map-sequence-id-73901

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  6. P25-pZE-SUMO-PbTTA

    Plasmid
    #204634
    Purpose
    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
    Depositor
    Aditya Kunjapur
    Article
    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:
    Insert
    His-SUMO-TEV-PbTTA
    Use
    Synthetic Biology
    Tags
    His Tag; SUMO Tag; TEV Protease Site
    Expression
    Bacterial
    Mutation
    Promoter
    PLtetO-1 promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204634-plasmid-map-sequence-id-414414

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  11. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid
    #105200
    Purpose
    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    105200-plasmid-map-sequence-id-201271

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  13. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid
    #107292
    Purpose
    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Dmytro Yushchenko
    Article
    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191
    Insert
    SNCA-GFP (SNCA Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107292-plasmid-map-sequence-id-209472

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  16. P14-pZE-SUMO-ObiH

    Plasmid
    #204629
    Purpose
    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
    Depositor
    Aditya Kunjapur
    Article
    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:
    Insert
    His-SUMO-TEV-ObiH
    Use
    Synthetic Biology
    Tags
    His Tag; SUMO Tag; TEV Protease Site
    Expression
    Bacterial
    Mutation
    Promoter
    PLtetO-1 promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204629-plasmid-map-sequence-id-414418

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  17. pBAD-bHs

    Plasmid
    #134259
    Purpose
    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag
    Depositor
    Edwin Manuel
    Article
    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7
    Insert
    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    codon optimized for Salmonella typhimurium
    Promoter
    AraC
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    134259-plasmid-map-sequence-id-264461

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  23. pET21-pelB-LaG-6

    Plasmid
    #172741
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-6
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172741-plasmid-map-sequence-id-340175

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  24. pHS1928

    Plasmid
    #205967
    Purpose
    JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    Cas8-HNH all proteins, with His-tagged Cas8
    Use
    Tags
    14xHis on Cas8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205967-plasmid-map-sequence-id-415395

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  36. MERS-coV-NSP3-c001

    Plasmid
    #173085
    Purpose
    Expression of recombinant fusion protein in E. coli
    Depositor
    Lari Lehtiö
    Article
    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012
    Insert
    rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
    Use
    Tags
    mCerulean
    Expression
    Bacterial
    Mutation
    D1109-D1275, codon optimized
    Promoter
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    173085-plasmid-map-sequence-id-349919

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  42. VIPR1

    Plasmid
    #51865
    Purpose
    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    VIPR1 (VIPR1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51865-plasmid-map-sequence-id-79760

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  45. pDual-eGFP(H66)

    Plasmid
    #63558
    Purpose
    Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(H66)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed tyrosine at position 66 with respect to t…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pET21-pelB-LaG-9

    Plasmid
    #172742
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-9
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172742-plasmid-map-sequence-id-340182

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  50. pET21-pelB-LaG-8

    Plasmid
    #172761
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-8
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172761-plasmid-map-sequence-id-340116

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Showing: 6401 - 6450 of 22079 results